Effect of secondary lung injury caused by mechanical trauma on the expression change of forkhead box protein Al at both mRNA and protein levels
- Author:
- No author available
- Journal Title:
- CHINA MEDICINE
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1673-4777.2011.06.011
- Key Word:
- 机械性创伤;急性肺损伤;叉头盒转录因子A1;Mechanical trauma;Acute lung injury;Forkhead box protein Al
Abstract： Objective To study the effect of secondary lung injury caused by mechanical trauma on the expression change of Forkhead box protein Al ( FoxAl) at both mRNA and protein levels. Methods Forty male Wistar rats were randomly divided into sham group and trauma group, each group 20, Noble-Collip drum was used to establish mechanical trauma rat model. Trie total RNA and protein of lung tissue was extracted after modeling of abdominal aortic blood sampling time points were killed. The expression of FoxAl mRNA in rats' lung tissue was observed by real-time RT-PCR, the expression of FoxAl protein was detected by Western Blot. Results The expression of FoxAl protein in trauma group was higher than that in sham group( sham group 0.59 ± 0.04, trauma group 0.91 ± 0.03, P < 0.01). Conclusion FoxAl plays an important role in secondary lung injury caused by mechanical trauma.
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