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The dynamic changes of serum tumor necrosis factor-α and the clinical significance in acute lung injury rats caused by mechanical trauma

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DAI Wen-juan(Department of Respiratory,Second Clinical Hospital,Shanxi Medical University ,Taiyuan 030001,China)
JIA Guo-ping(Department of Respiratory,Second Clinical Hospital,Shanxi Medical University ,Taiyuan 030001,China)
LIU Zhuo-la(Department of Respiratory,Second Clinical Hospital,Shanxi Medical University ,Taiyuan 030001,China)
GAO Xiao-ling(Department of Respiratory,Second Clinical Hospital,Shanxi Medical University ,Taiyuan 030001,China)
Journal Title:
Volume 06, Issue 04, 2011
Key Word:
Acute lung injury;Mechanical trauma;Tumor necrosis factor-α

Abstract: Objective To determine the dynamic changes and mechanism of tumor necrosis factor-α(TNF-α) in serum in acute lung injury(ALI) induced by mechanical trauma in mechanical trauma rat model. Methods Totally 40 male Wistar rats were randomly divided into sham group and trauma group, sham-acute lung injury group and acute lung injury group. Noble-Collip drum was used to establish mechanical trauma rat model. Intraperitoneal injection of TNF-α established acute lung injury model. All rats after modeling of abdominal aortic blood sampling time points were killed and the serum levels of TNF-α were asasyed by ELISA. Results Serum TNF-α levels (334. 78 ±± 28) ng/L in the trauma group were significantly higher than those in the sham group( 177 ±10 ) ng/L (P <0. 01 ). The pathological results showed that both in trauma group and acute lung injury group lung infection were very obvious, while it was basically normal in both sham group and sham-acute lung injury group. Conclusions Serum TNF-αt levels of mechanical trauma may be associated with acute lung injury after mechanical trauma. Therefore, dynamic observation of the change of serum TNF-α after mechanical trauma will help the assessment and prognosis of the disease, it has important clinical significance.

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