Your Position: Home > Journal List > CHINA MEDICINE > Paper

Pharmacokinetics of topically applied compound amphotericin B eye gel in rabbit eyes

( views:137, downloads:0 )
Author:
WU Wei(Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China)
HE Mei-feng(Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China)
TANG Xi-lan(Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China)
WANG Yan-dong(Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China)
LIANG Guang-jiang(Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China)
Journal Title:
CHINA MEDICINE
Issue:
Volume 05, Issue 12, 2010
DOI:
10.3760/cma.j.issn.1673-4777.2010.12.030
Key Word:
Amphotericin B;Rifampin;Pharmacokinetics;Gels

Abstract: Objective To study the intraocular penetration and pharmacokinetics of amphotericin B and rifampicin following topically applying compound amphotericin B eye gel in rabbit eyes. Methods Thirty rabbits were randomly divided into 10 groups. The tears were collected at 5, 15, 30, 45, 60, 90, 120, 180, 240 and 360 minutes after compound amphotericin B eye gel was applied to the rabbit eyes. Then the rabbits were sacrificed and the cornea and aqueous humors were collected immediately. The concentrations of amphotericin B and rifampicin were detected by High-performance liquid chromatography (HPLC) and pharmacokinetic parameters were estimated.Results Rifampicin could rapidly penetrate into rabbit ocular tissues, but amphotericin B in aqueous humors could not be detected by HPLC. Amphotericin B could maintain high concentration in tears for 4 hours. The pharmacokinetic parameters of amphotericin B and rifampicin in rabbits cornea were as follows: Tpeak was 20.96 and 7.57 min,Cmax was 3.32 and 25.37 μg/g, t1/2 was 45.34 and 31.69 min and AUC was 299.44 and 1368.91 min ·μg/g.Conclusions The results may provide experimental data for treatment of extraocular fungal infectious disease with compound amphotericin B eye gel. However, the therapeutic effect of compound amphotericin B eye gel on intraocular fungal infection need to be furtherly studied.

  • [1]EL-Azizi M.Enhancement of the in vitro activity of amphotericin B against the biofilms of non-albicans Candida spp.By rifampicin and doxycycline.J Med Microbiol,2007,56(5):645-649.
  • [2]刘瑞丽,李金伟,杜习智,等.罗红霉素眼用凝胶的研制.中国医药,2008,3(12):805-806.
  • [3]Lee SJ,Lee JJ,Kim SD.Topical and oral voriconazole in the treatment of fungal keratitis.Korean J Ophthalmol,2009,23(1):46-48.
  • [4]马文江,赵少贞,侯光华,等.真菌性角膜炎真菌鉴定及药物敏感实验分析.国际眼科杂志,2009,9(6):1157-1158.
  • [5]Yavas GF,Oztürk Fx,Oztürk F,et al.Antifungal efficacy of voriconazole,itraconazole and amphotericin B in experimental fusarium solani keratitis.Graefes Arch Clin Exp Ophthalmol,2008,246(2):275 -279.
  • [6]马小军,李桂萍,周炯,等.两性霉素B治疗侵袭性真菌感染回顾性研究.中华内科杂志,2007,46(9):718-720.
  • [7]Kaji Y,Yamamoto E,Hiraoka T,et al.Toxicities and pharmacokinetics of subconjunctival injection of liposomal amphotericin B.Graefes Arch Clin Exp Ophthalmol,2009,247(4):549-553.
  • [8]刘立春.真菌性角膜炎的实验室诊断及药物敏感试验分析.中国现代药物应用,2008,2(20):75.
  • [9]王建泉.眼部主要感染真菌对两性霉素B和氟康唑的敏感度.眼视光学杂志,2007,9(1):61,65.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn