Abstract： Objective To detect the expression of the FHIT and P14ARF protein in endometrial adenocarcinoma, endometrial atypical hyperplasia and the normal endometrium. To examine the relation of FHIT and P14ARF in the genesis and progression of endometrial carcinoma. Methods The expression of FHIT and P14ARF protein in 60 cases of endometrial adenocarcinoma. 30 cases of endometrial atypical hyperplasia and 30 cases of normal endometrial tissue were detected by SP immunohistochemical staining. Results The positive rates of FHIT in endometrial adenocarcinoma,endometrial atypical hyperplasia and normal endometrium were 53.3%, 63.3%, 100% respectively. The positive rates of FHIT in endometrial adenocarcinoma and endometrial atypical hyperplasia was significantly lower than that in normal endometrium,The difference was statistically significant (P ＜0.01 ). The positive rates of P14ARF in endometrial adenocarcinoma,endometrial atypical hyperplasia and normal endometrium were 56.7%, 73.3%, 90.0% respectively. The positive rate of P14ARF in endometrial adenocarcinoma was significantly lower than that in normal endometrium ( P ＜0.01 ). The positive expression of FHIT was associated with the surgical-pathologic staging of endometrial adenocarcinoma, organizational grade, myometrial invasion and lymph node metastasis (P ＜ 0.05 ). The positive expressions of P14ARF were only related to the organizational grade of endometrial adenocarcinoma (P ＜0.01 ). The positive expressions between FHIT and P14AR protein were of significant positive correlation in endometrial adenocarcinoma (rs = 0.396, P ＜ 0.01 ). Conclusions The expression of FHIT and P14ARF protein in endometrial adenocarcinoma,endometrial atypical hyperplasia and normal endometrium are gradually increased. In endometrial adenocarcinoma, the expression of FHIT are gradually decreased with surgical-pathologic stage, myometrial invasion and lymph node metastasis, they were of significant positive correlation between the two protein. The combined detection of FHIT and P14ARF protein has certain reference value in early diagnosis of endometrial adenocarcinoma and prognosis.