Abstract： Objective To study the distribution and drug resistance of pathogen in urinary system and to provide the credible evidence with the clinic diagnosis and the treatment of urinary diseases. Methods The pathogen from the urine specimen from the patients in the hospital were cultured and separated from January 2006 to December 2008.The total 923 strains of pathogen were identified and the drug resistance were analyzed. Results Escherichia coli among the pathogens which infects the urinary system rated the top,for 557(60.3%),secondly Enterococcus,Klebsiella,Acinetobacter Baumannii,Pseudomonas aeruginosa,and Fungis.The drug resistance of Escherichia coli to Imipenem was the lowest about 0.However,it was above 16.9% towards the most beta-lactam antibiotics;the drug resistance of Enterococcus was generally higher,but Vancomycin was lower about 10.9%. Conclusions The main pathogen is Enterobacteriaceac which infects the urinary system.The antibiotics pathogens should be chosen according to the test results of the pathogens sensitivity.