Your Position: Home > Journal List > CHINA MEDICINE > Paper

Influence of mineral water rich in microelement on proliferation of vascular smooth muscle cells

( views:, downloads: )
Author:
ZHAO Li-min(Beijing Anzhen Hospital of Capital Medical University & Beijing Institute of Heart Lung and Blood Vessel Diseases,Beijing 100029, China)
WEN Jie(Beijing Anzhen Hospital of Capital Medical University & Beijing Institute of Heart Lung and Blood Vessel Diseases,Beijing 100029, China)
LIU Kuo(Beijing Anzhen Hospital of Capital Medical University & Beijing Institute of Heart Lung and Blood Vessel Diseases,Beijing 100029, China)
LIU Jie-lin(Beijing Anzhen Hospital of Capital Medical University & Beijing Institute of Heart Lung and Blood Vessel Diseases,Beijing 100029, China)
LIU Ya(Beijing Anzhen Hospital of Capital Medical University & Beijing Institute of Heart Lung and Blood Vessel Diseases,Beijing 100029, China)
HONG Zhao-guang(Beijing Anzhen Hospital of Capital Medical University & Beijing Institute of Heart Lung and Blood Vessel Diseases,Beijing 100029, China)
TANG Chao-shu()
WEN Shao-jun(Beijing Anzhen Hospital of Capital Medical University & Beijing Institute of Heart Lung and Blood Vessel Diseases,Beijing 100029, China)
Journal Title:
CHINA MEDICINE
Issue:
Volume 5, Issue 03, 2010
DOI:
10.3760/cma.j.issn.1673-4777.2010.03.001
Key Word:
Microelement; Smooth muscle cells; Cell culture; Proliferation

Abstract: Objective To study the influence of mineral water rich in microelement on proliferation of human vascular smooth muscle cells(HVSMCs) in vitro. Method Ten samples of human great saphenous vein of patients undergoing coronary artery bypass surgery were obtained. Two kinds of DMEM media including Jinchuan mineral water (MW-DMEM)and double distilled water(DDW-DMEM) were observed. The free tissue formation time and the "peak-valley" time of HVSMCs cultured in these 2 kinds of DMEM media were observed and recorded.A cell proliferating model of HVSMCs was induced by angiotensin Ⅱ. 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny-ltetrazolium bromide (MTT) was used to determine the cells growth. The cell was confirmed to be HVSMCs by im-munohistochemistry and electromicroscopy analysis. Results The HVSMCs in MW-DMEM group emigrating from the tissue explanted within (3.7±0.2) days while the HVSMCs in DDW-DMEM group explanted within (8.5±0.6)days. The time of HVSMCs forming a 'peak-trough state' from a adherence state in MW-DMEM group was (8.3±0.2)days and it was (7.1±0.5)days in DDW-DMEM group. The result showed that H VSMCs proliferation induced by Ang Ⅱ could be inhibited by Jinchuan mineral water. HVSMCs were confirmed under electron microscope of the integrity of cell morphology and the surface is rich in microvilli. HVSMCs cytoplasm was rich in myofilaments and was in a longitudinal and parallel arrangement in intracellular dense areas Anti-α-actin antibodies with immuno-histochemical staining showed lots of stained buffy bundles in cell cytoplasm. Conclusions The results show that Jinchuan mineral water rich in microelement and mineral can relieve injury of HVSMCs. Meanwhile HVSMCs prolif-eration induced by Ang Ⅱ can be inhibited by Jinchuan mineral water.

  • [1]Tiwari A,Hamilton G,Seifalian AM,et al.Tissue engineering thera-py for cardiovascular disesae.Circ Res,2003,93(2):e1.
  • [2]Thormodsson FR,Olafsson IH.Isolation and culturing of human vas-cular smooth muscle cells.Methods Mol Biol,2005,299:197-210.
  • [3]鄂征.组织培养和分子细胞学技术.北京:北京出版社,1995:12.
  • [4]赵焕然.金川保健啤酒研究.北京:中国科学技术出版社,2005:4.
  • [5]舒晓亮,蔡东联.微量元素与动脉粥样硬化.肠外与肠内营养,2007,14(2):117-119,125.
  • [6]温绍君,许秀举,刘洁琳,等.饮用啤酒对高血压和/或高脂血症患者血压和血脂水平的影响.中国综合临床,2007,23(6):484-486.
  • [7]刘春宇,陈艳兰,石武祥.微量元素与心血管疾病关系的研究进展.现代预防医学,2008,35(1):67.
  • [8]M(u)ller DN.Bohlender J,Hilgers KF,et al.Vascular angiotensin-converting enzyme expression regulates local angiotensin Ⅱ.Hyper-tension,1997,29(1 pt 1):98-104.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn