Your Position: Home > Journal List > CHINA MEDICINE > Paper

The sensitization changes of SKOV3. ip1 cells exposed to taxel when the expression of Her-2 in the cells is depressed

( views:, downloads: )
Author:
WANG Ke-fang(Department of Obstetrics and Gynecology,Beijing Anzhen Hospital of the Capital University of Medical Sciences,Beijing 100029,China)
WANG Xian-feng()
Journal Title:
CHINA MEDICINE
Issue:
Volume 5, Issue 02, 2010
DOI:
10.3760/cma.j.issn.1673-4777.2010.02.001
Key Word:
Ovarian carcinoma;Her-2 gene;Taxel

Abstract: Objective To observe the sensitization changes of SKOV3. ip1 cells exposed to taxel when the expression of Her-2 in SKOV3. ip1 ceils was depressed. Meshods 1. Cell culture: SKOV3. ip1 cells and lower Her-2 expressed SKOV3. ip1 expressed SKOV3. ip1 cells was detected by Western blot. 3. MTT Test : SKOV3. ip1 cells were exposed to different dose taxel (6,12, 24,48,96 μg/ml), The MTT method was used to detect the inhibi-tion rate of SKOV3. ip1 cells exposed to taxel and the inhibition rate of SKOV3. ip1 cells that express different Her-2 level was compared. Results When exposed to(6,12, 24,48 μg/ml)taxel dose, the inhibition rate of lower Her-2 expressed SKOV3. ip1 cells were (45.50±0.95)%, (65.37±0.56)%, (76.83±0.27)% and (81.83±0.69)%. While the inhibition rate of SKOV3. ip1 cells were(36.13±1.74)%, (56.43±2.19)%, (67.57±1.96) % and(74.30±1.10) %. The difference were obviously (P < 0.01). Conclusion The sensitization of SKOV3. ip1 cells to taxel was increased when the expression of Her-2 in the cells was depressed.

  • [1]王克芳,崔恒,冯捷,等.BNA干扰技术抑制Her2基因表达对卵巢上皮性癌细胞生物学特性的影响.中华妇产科杂志,2008,43(8):622-625.
  • [2]Roninson IB.Molecular cellular biology of multidrug resistance in tumor cells.New York:Plenum Press.1991:396-398.
  • [3]Duan Z,Brakore KA,Selden MV.Inhibition of ABCB1 (MDR1) and ABCB4 (MDR3) expression by small interfering RNA and reversal of paclitaxcil resistance in human ovarian cancer cells.Mol Cancer Ther,2004,3 (7):833-838.
  • [4]邓凯贤,钟玲,姜梅贤,等.RNA干扰技术沉默survivin基因逆转卵巢癌细胞株SKOV3/ADM耐药性的研究.中华妇产科杂志,2005,40(12):836-839.
  • [5]Dodier P,Piche A.Bcl-X(L) is functionally non-equivalent for the regulation of growth and survival in human ovarian cancer cells.Gynecol Oncol,2006,100(2):254-263.
  • [6]Halder J,Landen CN Jr,Lutgendorf SK,et al.Focal adhesion kinase silencing augments docetaxel-mediated apoptosis in ovarian cancer cells.Clin Cancer Res,2005,11(24 Pt 1):8829-8836.
  • [7]Putral LN,Bywater MJ,Gu W,et al.RNA interference against human papillomavirus oncngones in cervical cancer cells results in increased sensitivity to cisplatin.Mol Pharmacol,2005,68 (5):1311-1319.
  • [8]Ueno NT,Bartholomeusz C,Hermann JL,et al.E1A-mediated paclitsxel sensitization in HER-2/neu-overexpressing ovarian cancer SKOV3.ip1 through apoptosis involving the caspase-3 pathway.Clin Cancer Res,2000,6(1):250-259.
  • [9]任淑华,王井伟,张林.Her-2/neu小分子干扰RNA对肺腺癌细胞周期和凋亡机制的影响.中华医学杂志,2005,85(22):1530-1534.
  • [10]Yang G,Cai KQ,Thompson-Lanza JA,et al.Inhibition of brenst and ovarian tumor growth through multiple signaling pathways by using retrovirus-mediated small interfering RNA against Her-2/neu gene expression.J Biol Chem,2004,279(6):4339-4345.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn