Abstract： Objective To assess human papillomavirus(HPV)detection method in screening cervical intra-epithelial neoplasia and cervical cancer.Methods Totally 358 clinical cases were enrolled.Three screening methods included HPV DNA testing,thinprep cytologic test(TCT)and multiple punch biopsy by colposcopy.The pathology results were regarded as gold standard.Results The sensitivity(Se),specificity(Sp),efficiency(E),positive predictive value(PV+),negative predictive value(PV-),Youden,s index(Y)value of each diagnostic test were:TCT(66.28%,94.49%,87.71%,79.17%,89.86%,60.77%),HPV DNA testing(81.40%,83.82%,82.24%,61.40%,93.44%,65.22%),colposcopic evaluation(72.62%,92.70%,87.99%,75.30%,91.70%,60.61%).The sensitivity of HPV DNA testing was the highest(81.40%)and TCT was the lowest(66.28%).There was a statistically significant diffierence between HPV DNA testing and TCT(P<0.05).Conclusions HPV DNA testing has a very high sensitivity for the detection of CINⅡ.TCT has a high specificity.which can reduce the misdiagnosis rate and omission diagnostic rate by combining the colposcopic evaluation and biopsy test.HPV method is a good mothod of screening ASCUS.