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Two different methods for repeated intrathecal administration in rats

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Author:
PEN Zhon-jie(Department of Anesthesiology, Weifang Medical College, Weifang 261042, China)
YU Zhi-jun()
ZHANG Zeng-zhen(Department of Anesthesiology, Weifang Medical College, Weifang 261042, China)
ZHANG Cheng-ming(Department of Anesthesiology, Weifang Medical College, Weifang 261042, China)
ZHANG Gnang-xue()
Journal Title:
CHINA MEDICINE
Issue:
Volume 4, Issue 06, 2009
DOI:
10.3760/cma.j.issn.1673-4777.2009.06.034
Key Word:
Spinal injections; Subarachnoid space; Catheters indwelling; Punctures

Abstract: Objective To compare the feasibility of direct spinal puncture and reserving micro-catheter in spinal space during repeated intrathecal administration. Methods Forty Sprague-Dawley rats were randomly divided into 2 groups:direct spinal puncture (group puncture) and reserving micro-catheter in spinal space group (group reserve). The duration of operation were recorded. 5 days after operation, rata in both groups were intrathecally administerde morphine(10 μg/10μl) for 7 days and thermal withdrawal latency(TWL) were assessed. Results The operation in group reserve took shorter time than the group puncture, but micro-catheter were pulled out in 5 rats in group reserve. There is no difference of TWL between two groups. Conclusion Both methods are suitable for repeated intrathecal administration.

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