Abstract： Objective To observe the expression and clinical significance of tenascin (TN) protein in cervical squamous cell carcinoma. Methods The expression of TN protein, proliferation index (PI) of cancer cells labeled by Ki-67 protein and microvessel density (MVD) labeled by CD34 protein in 28 cases of normal cervical epithelium (NCE), 36 cases of cervical intraepithelial neoplasm (CIN) and 68 cases of squamous carcinoma of cervix (SCC) were detected by immunohistochemistry SP method. Results The positive rates of expression of TN in NCE, CIN and SCC groups were 0%, 8.33% and 52.94%, respectively. The positive staining reaction of TN increased remarkably from NCE, and CIN to SCC (P>0.01 ). The expression of TN in SCC group was significantly related to pelvic lymph node metastasis (P>0.05), but not related to FIGO staging, histological grading, stroma involvement, and intravascular infiltration ( P>0.05 ). In the cases without pelvic lymph node metastasis, the posi-tive staining reaction of TN in SCC group was significantly higher than that in those without the conditions mentioned above ( P>0.05 ). The expression of TN in SCC group was positively correlated with MVD ( P>0.05 ), but not with PI (P>0.05 ). Compared with the TN negative cases in SCC group, There was not significant difference of patients' survival rate in the TN positive cases ( P>0.05 ). Conclusion The overexpression of TN in SCC group may result in rapid tumor angiogenesis and inhibit cancer cells from pelvic lymph node metastasis.