Abstract： Objective To measure serum IL-6 and IL-10 in acute painful rats of intratheeal butorphanol and to investigate theraputic mechanisms of butorphanol on acute pain. Methods Fourty-five healthy male SD rats were randomly divided into three groups. Every group was equally divided into three subgroups with5 animals in each subgroup. Group B accepted the same basic anesthesia without any operation and blood was obtained from epieathus of rats. Group C received intrathecal normal saline 10 μl after CCI. Group T received intrathecal butorphanol 24 μg after CCI. In subgroup 1 blood samples were drawn from epicathus before the CCI, 9 hours, 18 hours after CCI. Similarly, blood samples in subgroup 2 were obtained at hours 3, 12, 24 while subgroup3 on hour6, 15,48, respectively. Serum IL-6 and IL-10 in each sample were measured by enzyme linked immunosorbent assay. Results Serum IL-6 in group C and group T within 48 hours after CCI increased after operation. In group C, the peak time of serum IL-6 was 6 hours after operation with 12 hours in group T. The serum IL-6 in group T were lower than that in group C at the same time point. Serum IL-10 in group C began to increase 12 hours after CCI. Serum IL-IO production in group C reached peak value at 18h after CCI, with 15h in group T. Conclusion Intratheeal butorphanol decreases the level of serum IL-6 and increases serum IL-10 ,accelerating the balance of promotion or inhibitor inflammatory factors.