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Molecular mechanism of DU145 prostatic cancer cells apoptosis induced by 13-MTD

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Author:
No author available
Journal Title:
CHINA MEDICINE
Issue:
7
DOI:
10.3760/cma.j.issn.1673-4777.2008.07.004
Key Word:
前列腺癌;13-甲基十四烷酸;细胞凋亡;Prostate cancer;13-methyltetradecanoic acid;Apoptosis

Abstract: Objective To investgate the molecular mechanism of DU145 prostatic cancer cells apoptosis induced by 13-MTD. Methods Prostate carcinoma cell line DU145 was treated with solvent or 13-MTD at 140 μg/ml for 4h, 8h and 24h. Cytosol Cytochrome C released from mitochomdria, cleavage of PARP, Lamin B and Rb proteins were analysed by Western blot . Caspase-dependent analysis were performed with inhibitor z-DEVD-fmk and z-VAD-fmk. 3 kinds of normal epithelial cell lines were treated with 13-MTD compared with DU145, released Cytochrome C and cleavaged of Lamin B were detected by western blot. Result After treatment with 140μg/ml 13-MTD, cytosol Cytochrome C released from mitochondrion could be detected by Western blot, indicating mitochondrion attended the 13-MTD-induced apoptotic in DU145 prostatic cancer cells, which could not be eliminated by Caspase inhibitor z-DEVD-fmk and z-VAD-fmk. Fragmentation of PARP, Rb and Lamin B were detected in the 13-MTD-induced prostatic cancer cells, but not in the 13-MTD-induced normal cells, showing that 13-MTD selectively induced apoptosis for cancer cells and had no effect on normal cells. This reaction could be inhibited by z-DEVD-fmk and z-VAD-fmk, indicating that this 13-MTD-induced apoptotic in DU145 prostatic cancer cells was caspase -dependent. Conclusion 13-MTD induces apoptosis of prostate cancer cell line DU145, not normal human epithelial cell, through mitochondria-attended and Caspase-dependent pathway.

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