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Influence of RNA interference of Vascular endothelial growth factor-C on treatment of breast cancer in nude mice
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- Author:
- No author available
- Journal Title:
- CHINA MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1673-4777.2008.02.002
- Key Word:
- 乳腺癌;血管内皮生长因子-C;短发夹RNA
Abstract： 目的 检测血管内皮生长因子-C(VEGF-C)的RNA干扰技术对裸鼠乳腺癌的影响作用,探讨RNA干扰对乳腺癌基因治疗中的有效性和相关机制.方法 36只NOD-SCID小鼠随机分为空载体组12只,阴性短发夹RNA(ShRNA)对照组12只及VEGF-CShRNA转染组12只.构建携带有绿色荧光蛋白报告基因的VEGF-CShRNA质粒载体,并导入乳腺癌细胞MCF-7裸鼠皮下移植瘤,尾静脉注射VEGF-CShRNA表达载体,观察其对乳腺癌生长的影响作用;酶联免疫吸附剂测定(ELISA)方法检测VEGF-C在肿瘤组织中的蛋白浓度;免疫组织化学分析肿瘤组织VEGFR-3阳性脉管密度.结果 全身系统性给予VEGF-CShRNA后,空载体组、阴性ShRNA对照组及VEGF-C ShRNA转染组肿瘤体积分别为(250±4)mm3、(230±26)mm3 (P=0.2)、(97±25)mm3 (P＜0.05);瘤组织中VEGF-C浓度在空载体组、阴性ShRNA对照组及VEGF-CShRNA转染组分别为(496±7)pg/mg、(362±95)pg/mg(P=0.2)和(115±55)pg/mg(P＜0.05);免疫组化分析VEGF-C ShRNA转染组肿瘤组织中VEGFR-3阳性脉管生成减少(P＜0.05).结论 VEGF-C的RNA干扰明显抑制了VEGFR-3阳性脉管的形成,抑制肿瘤的生长,有望通过RNA干扰技术实现乳腺癌的基因治疗.
- [1]杨小青,徐卫国.Neuropilins与肿瘤相关性的研究进展[J].2006,(8).
- [2]王刚,代小菊,徐卫国,等.血管内皮生长因子与肿瘤的抗血管生成治疗[J].2006,(9).
- [3]A H, Lee,E A, Dublin,L G, Bobrow,etc.Invasive lobular and invasive ductal carcinoma of the breast show distinct patterns of vascular endothelial growth factor expression and angiogenesis.[J].1998,185(4).
- [4]van Hinsbergh VW,Kaipainen A,Dumont D,etc.Expression of the fms-like tyrosine kinase 4 gene becomes restricted to lymphatic endothelium during development.[J].1995,92(8).
- [5]Plate K.From angiogenesis to lymphangiogenesis.[J].2001,7(2).
- [6]Liu W,Parikh A,Ahmad SA,etc.Role of angiogenesis in the development and growth of liver metastasis.[J].2002,9(7).
- [7]T, Niki,S, Iba,M, Tokunou,etc.Expression of vascular endothelial growth factors A, B, C, and D and their relationships to lymph node status in lung adenocarcinoma.[J].2000,6(6).
- [8]Yoshihara M,Kitadai Y,Furudoi A,etc.Clinical significance of vascular endothelial growth factor C expression and angiogenesis at the deepest invasive site of advanced colorectal carcinoma.[J].2002,62(2).
- [9]Alitalo K,Koji T,Tsurusaki T,etc.Vascular endothelial growth factor-C expression in human prostatic carcinoma and its relationship to lymph node metastasis.[J].1999,80(1).
- [10]Tilo, Kunath,Gerald, Gish,Heiko, Lickert,etc.Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype.[J].2003,21(5).