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Protective Effect of Shen-fu parenteral solution on intestinal epithelial cell in hemorrhagic shock rats
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- Author:
- No author available
- Journal Title:
- CHINA MEDICINE
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1673-4777.2007.08.006
- Key Word:
- 失血性休克;参附注射液;肠上皮细胞;线粒体膜电位
Abstract： 目的 探讨参附注射液对失血性休克后大鼠肠道损伤的保护作用及机制.方法 采用失血性休克模型,将雄性Wistar大鼠80只随机分为正常对照组、休克对照组、参附治疗组及单纯复苏对照组4组各20只.取失血性休克模型的远端回肠约40 cm,用紫外分光法测定上述各组肠黏膜组织中过氧化物歧化酶(SOD)活性及丙二醛含量.用常规方法分离肠上皮细胞,以激光共聚焦显微镜检测肠上皮细胞膜电位值的变化.结果 与失血性休克组比较,参附注射液可以明显提高试验大鼠肠黏膜组织中SOD活性并显著降低丙二醛含量(P＜0.05);与休克和单纯复苏组比较,参附组荧光强度显著增强,有统计学意义(P＜0.01,P＜0.05).结论 参附注射液对失血性休克大鼠肠道具有保护作用.
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