Abstract： Objective　To clarify the effects of carbon disulfide(CS2) on hydroxyl free radical(*OH) generation in phenanthroline chemiluminescence system(Phen-system). Methods　The dynamic curve of various concentrations of CS2,the quenching effect of thiourea on chemiluminescence intensity and the influence of CS2 on Phen-system without CuSO4 and Vit C were observed with phenanthroline chemiluminescence system. Results　CS2 could enhance the emission intensity of chemiluminescence and advance the peak time.It had dose-effect relationship between CS2 concentrations (0,10,40,80,120,160 mg/ml) and chemiluminescence peak (r=0.955,R2=0.913，P=0.003),as well as CS2 concentrations and peak time(r=-0.927，R2=0.860，P=0.008).The enhanced chemiluminescence induced by CS2 could be quenched by thiourea but the inhibition rate of it reduced along with the increasing of CS2　concentration(r=-0.816，R2=0.666，P=0.048).When the system were free of CuSO4 and Vit C,CS2 could also induce the emission of luminous intensity. Conclusion　CS2 could induce the generation of hydroxyl free radicals and advance the peak time in Phen-system.