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Preliminary study on erythrocyte membrane spectrin in workers exposed to carbon disulfide
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- Author:
- No author available
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1001-9391.2000.05.003
- Key Word:
- 二硫化碳;十二烷基硫酸钠-聚丙烯酰胺凝胶电泳;收缩蛋白;生物标志物
Abstract： 目的 观察作业工人长期接触二硫化碳 (CS2)对其红细胞膜收缩蛋白的影响.方法 气相色谱法测定作业场所CS2浓度;分离16名工人红细胞膜(接触、对照各8人),采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法分析红细胞膜蛋白谱.结果 车间空气 CS2浓度(TWA)为0～8.98 mg/m3.CS2接触工人红细胞膜收缩蛋白百分含量(34.48%)高于对照工人(26.18%)(t=5.920,P＜0.001),CS2浓度(TWA)与收缩蛋白含量呈正相关(r=0.859,R2=0.738,P＜0.001),接触指数(EI)与收缩蛋白含量也呈正相关(r=0.713,R2=0.508,P=0.002).其中2名长工龄高浓度CS2接触工人红细胞膜图谱在高相对分子质量区有可疑异常区带形成.结论 长期接触CS2作业工人红细胞膜蛋白电泳图谱出现异常,提示该指标有希望成为CS2接触工人的分子水平接触-效应生物标志物.
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