Abstract： Objective To investigate the distribution and drug resistance of the major pathogens in infectious ophthalmic diseases.Methods A total of 2397 specimens of infectious ophthalmic diseases in Tianjin Eye Hospital during January 2009 to December 2011 were cultured and identified for bacteria,fungus and Acanthamoeba.The distribution and drug resistance of pathogens were analyzed.Results Of the 2397specimens,799 (33.33％) were positive in culture,in which 701 (87.73％) were positive for bacteria,95 ( 11.89％ ) were positive for fungus,and 3 (0.36％) were positive for Acanthamoeba.In 701 bacterial isolates,533 (76.03％ ) were Gram-positive cocci and Staphylococcus epidemidis was the predominant (46.15％,246/533 ) ; 138 ( 19.69％ ) isolates were Gram-negative bacillus,in which nonfermenters were the most common including 40 (28.98％) Pseudomonas aeruginosa strains; the rest 30 (4.28％) isolates were Gram-positive bacillus.Of the 95 fungus isolates,93 ( 97.89％ ) were filamentous fungi including fusarium (54/95,56.84％),alternaria (13/95,13.68％) and aspergillus (17/95,17.88％).In vitro drug sensitivity tests showed that Staphylococcus epidemidis was completely sensitive to vancomycin and minomycin ; Pseudomonas aeruginosa was 100％ resistant to ampicillia/sulbactam, compound sulfamothoxazole and cefotaxime,and was completely sensitive to amikacin,polymyxin E,meropenem,piperacllin/tazobactam and tobramycin; Fusarium solani was of high sensitivity to amphotericin B.Concluslons Bacteria especially Gram-positive cocci are the most common pathogens in infectious ophthalmic diseases,and they are highly sensitive to vancomycin.Fusarium is the most common fungal pathogen,which is sensitive to amphotericin B.