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In vitro effect of human amniotic fluid-derived mesenchymal stem cells on Th1/Th2 ratio in peripheral blood of hepatitis B patients with liver failure
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Clinical Infectious Diseases
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1674-2397.2012.03.005
- Key Word:
- 肝功能衰竭;肝炎,乙型;羊水间充质干细胞;Th1-Th2平衡
Abstract： 目的 探讨人羊水间充质干细胞(human amniotic fluid-derived mesenchymal stem cells,h-AFMSC)对乙型肝炎肝衰竭患者外周血Th1/Th2的体外调节作用.方法 选择2011年2-5月中山大学附属第三医院6例乙型肝炎肝衰竭患者和6名健康志愿者,提取外周血单个核细胞( peripheralblood mononuclear cells,PBMC),同时从孕妇中期羊水中提取h-AFMSC.将h-AFMSC分别与乙型肝炎肝衰竭患者和健康捐献者的PBMC共培养72 h,乙型肝炎肝衰竭患者和健康捐献者的PBMC单独培养组作为对照.应用流式细胞仪检测共培养前后不同组PBMC IFNγ(Th1)和IL-4(Th2)的变化情况,计算Th1/Th2比值.对共培养前后数据进行Mauchly球形检验,P＞0.1满足球形假设检验则采用重复测量方差分析.结果 共培养72 h,乙型肝炎肝衰竭患者PBMC与h-AFMSC共培养组的Th1细胞比例与其PBMC单独培养组比较无明显差异(F=0.82,P＞0.05),但Th2细胞比例明显下降(F=10.24,P＜0.05),从而导致Th1/Th2比值显著上升(F=17.28,P＜0.01).健康捐献者PBMC与h-AFMSC共培养组的Th1、Th2和Th1/Th2比值与其PBMC单独培养组比较差异均不明显(F值分别为0.54、0.68和0.74,P值均＞0.05).结论 h-AFMSC对乙型肝炎肝衰竭患者的Th1/Th2有体外调节作用,具有作为移植种子来源移植治疗乙型肝炎肝衰竭的可能性.
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