Abstract： Objective To investigate the infection and risk factors of multidrug-resistant Pseudomonas aeruginosa in diabetic foot.Methods Totally 85 strains of Pseudomonas aeruginosa were isolated from 428 samples of diabetic foot ulcers in Tianjin Metabolic Diseases Hospital from Jan 2008 to Dec 2010.Drug sensitivity tests were performed and multivariate logistic regression analysis was used to examine the risk factors of multidrug-resistant Pseudomonas aeruginosa infection. Results In 85 strains of Pseudomonas aeruginosa, 28 (32.94％ ) were multidrug resistant. Multidrug-resistant Pseudomonas aeruginosa showed high resistance to 3rd generation cephalosporins,quinolones and aminoglycosides with resistant rates of 42.86％-67.86％,32.14％ -57.57％,and 42.86％-67.86％,respectively. Logistic analysis indicated that previous antibiotic treatments, hypertension and anemia were associated with multidrug-resistant Pseudomonas aeruginosa infection ( OR =5.758,0.257 and 0.270,P =0.006,0.014 and 0.013 ). Conclusion Multidrug-resistant Pseudomonas aeruginosas isolated from diabetic foot are highly resistant to several commonly used antibiotics,and previous antibiotics use,hypertension and anemia are risk factors for multidrug-resistant Pseudomonas aeruginosa infections.