Flow cytometry method for antifungal susceptibility test and its clinical application

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Author:
XU Rui-long(Clinical Laboratory, Jinhua Central Hospital, Jinhua 321000, Zhejiang Province, China)
ZHU Yi-jun(Clinical Laboratory, Jinhua Central Hospital, Jinhua 321000, Zhejiang Province, China)
BO Li-hong(Clinical Laboratory, Jinhua Central Hospital, Jinhua 321000, Zhejiang Province, China)
XU Jian-bo(Clinical Laboratory, Jinhua Central Hospital, Jinhua 321000, Zhejiang Province, China)
PAN Yun(Clinical Laboratory, Jinhua Central Hospital, Jinhua 321000, Zhejiang Province, China)
SHAN Xiao-yun(Clinical Laboratory, Jinhua Central Hospital, Jinhua 321000, Zhejiang Province, China)
ZHENG Zhao-jing(Clinical Laboratory, Jinhua Central Hospital, Jinhua 321000, Zhejiang Province, China)
WU Jun-qing()
WANG Hui-jiao()
Journal Title:
Chinese Journal of Clinical Infectious Diseases
Issue:
Volume 04, Issue 06, 2011
DOI:
10.3760/cma.j.issn.1674-2397.2011.06.005
Key Word:
Flow cytometry;Fungi;Microbial sensitivity tests

Abstract: Objective To develop a rapid detection method of flow cytometry for antifungal susceptibility testing.Methods The experiment conditions of flow cytometry were optimized and the minimum inhibitory concentrations (M1Cs) of 230 strains of Candida spp to flucytosin,fluconazol,itraconazole,amphotericin B were detected.The MIC results obtained by flow cytometry were compared with M27-A2 reference assay.Results The agreement of MIC results between flow cytometry and M27-A2method was 85.0% - 100%.Conclusion Flow cytometry can be used as a rapid and reliable method for antifungal susceptibility testing.

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