HIV-1 drug-resistance mutations in treatment-na(i)ve patients in China

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Author:
YIN Chun-yu(Department of Infectious Diseases, the Sixth People's Hospital of Hangzhou , Hangzhou 310014, Zhejiang Province, China)
LU Hong-zhou()
HUANG Xiao-xiao(Department of Infectious Diseases, the Sixth People's Hospital of Hangzhou , Hangzhou 310014, Zhejiang Province, China)
LI Xiao-ou(Department of Infectious Diseases, the Sixth People's Hospital of Hangzhou , Hangzhou 310014, Zhejiang Province, China)
LOU Guo-qiang(Department of Infectious Diseases, the Sixth People's Hospital of Hangzhou , Hangzhou 310014, Zhejiang Province, China)
WENG Xin-hua()
Journal Title:
Chinese Journal of Clinical Infectious Diseases
Issue:
Volume 04, Issue 04, 2011
DOI:
10.3760/cma.j.issn.1674-2397.2011.04.004
Key Word:
Human immunodeficiency vires 1;Drug resistant;Mutation

Abstract: Objective To investigate the prevalence of drug-resistant mutations in reverse transcriptase and protease coding regions of HIV-1 in treatment-na(i)ve patients. Methods Plasma specimens were collected from 88 patients from Zhejiang, Shanghai, Henan and Anhui. The entire protease gene and the first 1-251 amino acids of the reverse transcriptase gene were amplified by RT-PCR from viral RNA and sequenced. The sequences were analyzed with HIV drug resistance algorithm, and phyligenetic analyses were performed by PHYLIP software. SPSS 13.0 was used for statistical analysis, and Fisher' s exact test was performed to compare the proportions of each subtype between the groups. Results 79 gene sequences were obtained, subtyping analyses indicated that 68.4% (54/79) were subtype B, followed by CRF01 _AE 24.8% (22/79), CRF07_BC 2.5% (2/79), and CRF08_BC 1.3% (1/79). 7 (7/79, 8.9%)presented with primary mutations associated with resistance to antiretroviral drugs, mutations conferring primary resistance to nucleoside reverse transcriptase inhibitors (NRTIs) and non-nucleoside reverse transcriptase inhibitors ( NNRTIs ) were detected in 3 ( 3. 8% ) and 4 ( 5. 1% ) cases, respectively. Protease inhibitors (Pls) associated primary resistance mutations were not found. Conclusion Antiretroviral drug resistant mutations have been found in treatment-na(i)ve patients with HIV-1 infections, while the prevalence level is low, which indicates that drug resistance test is not necessary for most HIV-1infected treatment-na(i)ve patients.

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