Detection and typing of HPV DNA in clinical specimens by polymerase chain reaction assays
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1674-2397.2011.02.003
- Key Word:
- 乳头状瘤病毒感染;聚合酶链反应;多态性,限制性片段长度;基因测序;分型;Papillomavirus infections;Polymerase chain reaction;Polymorphism,restriction fragment length;Gene sequencing;Typing
Abstract： Objective To assess polymerase chain reaction(PCR)combined with restriction fragment length polymorphism(RFLP)and gene sequencing technologies in the detection and typing of HPV DNA.Methods Tissue specimens were collected from skin diseases and venereal disease in perianal or genitals.PCR was performed with HPV DNA general primers(MY09/11)in tissue samples. Positive fragments of HPV DNA were purified and digested by restriction enzymes.The digested fragments were typed by po]yacrylamide gel electrophoresis(PAGE).The Results were verified by direct sequencing.Results In 50 clinical samples there were 35 HPV DNA positive,including 26 from patients with condyloma acuminatum,8 from patients with bowenoid papulosis,and 1 from patients with squamous cell carcinoma.In HPV DNA positive samples,19 were HPV6,3 were HPV11,8 were HPV16,4 were HPV6 and HPV 11,and I was HPV62.Sequencing Results were in accordance with the PCR-RFLP Results .Conclusion PCRRFLP method is effective in the detection and typing of HPV DNA.
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