Association of polymorphisms of -139 and -336 nucleotides in DC-SIGN promoter region with HIV infection

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Author:
LI Qin-guang(Institute of Liver Diseases, Fuzhou Hospital of Infectious Diseases, Fujian Medical University, Fuzhou 350025, China)
XU Li-jun(Institute of Liver Diseases, Fuzhou Hospital of Infectious Diseases, Fujian Medical University, Fuzhou 350025, China)
ZHANG Qi-yun(Institute of Liver Diseases, Fuzhou Hospital of Infectious Diseases, Fujian Medical University, Fuzhou 350025, China)
HUANG Fan()
CHEN Hui-cong(Institute of Liver Diseases, Fuzhou Hospital of Infectious Diseases, Fujian Medical University, Fuzhou 350025, China)
CHEN Rong-hua(Institute of Liver Diseases, Fuzhou Hospital of Infectious Diseases, Fujian Medical University, Fuzhou 350025, China)
Journal Title:
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
Issue:
Volume 03, Issue 04, 2010
DOI:
10.3760/cma.j.issn.1674-2397.2010.04.004
Key Word:
Human immunodeficiency virus;Dentritic cell specific intercellular adhesion molecule-3-grabbing nonintegrin;Susceptibility;Infection;Lymphocyte

Abstract: Objective To investigate the polymorphisms of-139 and -336 nucleotides in dendritic cells specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) promoter region in context of HIV susceptibility, infection routines and HIV/AIDS progress. Methods Polymorphisms of -139 and -336 nucleotides in DC-SIGN were examined in 160 HIV-positive subjects and 178 healthy controls;the Spearman test was performed to analyze their associations with HIV infection status. Results In 160 HIV-positive subjects, there were 92 (57.5%) with-139C, 68 (42.5%) with-139T, 29 (18.1%) with-336C and 131 (81.9%) with -336T. The frequencies of -139T/C and -336T/C in HIV-positive subjects were similar to those in the healthy controls (χ2 =0. 121 and 1. 754, P >0.05 ). No differences were found in the distribution of -139T/C or -336T/C in HIV-positive subjects infected via sex intercourse or intravenous drug (χ2 =0. 435 and 0. 103, P > 0. 05 ). -139C was usually companied with -336C ( r = 0. 359, P < 0.01 ).-139T (27.9%) were more frequently presented in patients with CD4 +T cells ≤50 cells/μL than -139C( 23.0%, χ2 = 4.055, P < 0.05 ). -139T/C and -336T/C were not related to HIV RNA levels ( t = - 0. 643and - 1. 637, P > 0.05). Conclusions Genotype -139C in DC-SIGN promoter region usually coexist with -336C. Polymorphisms of -139 and -336 are not related to HIV susceptibilities or HIV infection routes.-139T genotype may be related to serious depletion on CD4 + T cells.

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