Carbapenem resistance of KPC-possessing Enterobacteriaceae

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HONG Xiao-ping(Department of Clinical Laboratory,Centre for Occupational Diseases Prevention and Treatment,State Power Grid Company,Zhejiang Province,Jiande 311600,China)
YANG Qing()
Journal Title:
Volume 3, Issue 03, 2010
Key Word:
Microbial sensitivity testirig;Carbapenemase;Drag resistance

Abstract: Objective To evaluate 5 drug sensitivity tests in detecting carbapenem resistance of KPC-possessing Enterobacteriaceae.Methods Thirty-six Enterobacteriaceae isolates were collected from the First Affiliated Hospital,Cofiege of Medicine,Zhejiang Univeristy,including 32 of Klebsiella pneumoniae,1 of Escherichia coli,1 of Citrobacter freundii,1 of Klebsiella oxytoca,and 1 of Serratia marcescens,and the cacbapenemase producing was confirmed with PCR.Resistance to imipenem,meropenem and ertapenem was determined by agar dilution and disk diffusion tests,that to imipenem and meropehem was determined by ATB with G-5 panel,that to imipenem by BD Phoenix 100 with NMIC-109 panel,and that to imipenem and ertapenem by VITEK 2 Compact with AST-GN13 card.Results The resistance to imipenem determined by ATB,BD Phoenix100,VITEK 2 Compact,agar dilution and disk diffusion tests were 13.9%(5/36),11.1%(4/36),13.9%(5/36),22.2%(8/36)and 69.4%(25/36).ATB,agar dilution and disk diffusion tests reported 22.2%(8/36).55.6%(20/36)and 47.2%(17/36)as resistant to meropenem.VITEK 2 Compact,agar dilution and disk diflusion tests reported 69.4%(25/36),77.8%(28/36)and 88.9%(32/36)as resistant to ertapenem.The MICs of ertapenem determined by VITEK 2 Compact and agar dilution were≥2 μg/mL.Conclusion The sensitivity of agar dilution and agar disk diffusion is higher than other automatic methods in the identification of KPC-mediated carbapenem resistance,and ertapenem is a more sensitive indicator than mempenem and imipenem for screening KPC-possessing isolates.

  • [1]叶素娟,杨青,俞云松.2005年中国CHINET大肠埃希菌和肺炎克雷伯菌耐药性分析.中国感染与化疗杂志,2007,7(4):283-2s6.
  • [2]Yigit H,Queenan AM,Anderson GJ,et al.Novel carbapenemhydrolyzing beta-laetamase,KPC-1,from a carbapenem-resistant strain of Klebsiella pneumoniae.Antimierob Agents Chemother,2001,45(4):1151-1161.
  • [3]Woodford N,Tiemo PM Jr,Young K,et al.Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A beta-lactamase,KPC-3,in a New York Medical Center.Antimierob Agents Chemother,2004,48(12):4793-4799.
  • [4]Yigit H,Queenan AM,Rasheed JK,et al.Carbapenem-resistant strain of Klebsiella oxytoea harboring carbapenem-hydrolyzing β-lactamase KPC-2.Antimierob Agents Chemother,2003,47(12):388l-3889.
  • [5]Wei ZQ,Du XX,Yu YS,et al.Plasmid-mediated KPC-2 in a Klebsiella pneumoniae isolate from China.Antimierob Agents Chemother,2007,51(2):763-765.
  • [6]Bratu S,1Vlooty IVl,Niehani S,et al.Emergence of KPC-possessing Klebsiella pneumoniae in Brooklyn,New York:epidemiology and recommendations for detection.Antimicrob Agents Chemother,2005,49(7):3018-3020.
  • [7]Cai JC,Zhou HW,Zhang R,et al.Emergence of Serratia marcescens,Klebsiella pneumoniae,and Eseherichia coli isolales possessing the plasmid-mediated carbapenem-hydrolyzing betaJactamase KPC-2 in intensive care units of a Chinese hospital.Antimierob Agents Chemother,2008,2(6):2014-2018.
  • [8]Anderson KF,Lonsway DR,Rasheed JK,et al.Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobaeteriaceae.J Clin Microbiol,2007,45(8):2723-2725.
  • [9]Tenover FC,Kalsi RK,Williams PP,et al.Carbapenem resistance in Klebsiella pneumoniae not detected by automated susceptibility testing.Emerg Infect Dis,2006,12(8):1209-1213.
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