Smac/DIABLO and cytochrome c mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatocytes

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Author:
WANG Hong-jiao(Department ofInfectious Diseases,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,China)
XU Zhi-wei(Department ofInfectious Diseases,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,China)
CHEN Jun-ya(Department ofInfectious Diseases,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,China)
LIN Hai-long(Department ofInfectious Diseases,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,China)
CHEN Yi-ping(Department ofInfectious Diseases,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,China)
CHEN Yong-ping()
Journal Title:
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
Issue:
Volume 02, Issue 06, 2009
DOI:
10.3760/cma.j.issn.1674-2397.2009.06.006
Key Word:
Cytochromes c; Second mitochondria-derived activator of caspase;Hepatocyte transplantation; Microencapsulated hepatocyte

Abstract: Objective To investigate Smac/DIABLO and cytochrome c(cyt-c)mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatocyte.Methods Acute hepatic failure were induced by intraperitoneal injection of D-galactosamine in rats.and the rats were treated with microencapsulated hepatocytes,free hepatocytes and physical saline(contr01),respectively.Smac/DIABLO and cyt-c mRNA in liver tissue was detected by RT-PCR and the mRNA expression levels among three groups were compared.Results Smac/DIABLO and cyt-c mRNA levels in liver tissues of rats with acute hepatic failure were higher than those of normal rata(F=4.345,14.821,47.565,42.178 and 62.961,P<0.05).The peak values of Smac/DIABLO and cyt-C mRNA expressions in free hepatocytes and control groups were at 48 h.while that in microencapsulated hepatoeytes group was at 24 h.Conclusion Smac/DIABLO and cyt-c mRNA expression is an indicator of apoptosis of hepatocytes.

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