IL-1βand IL-1ra contents in cerebrospinal fluid of children with Japanese encephalitis

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Author:
LI Wei(Department of Pediatrics,Yuying Children's Hospital Affiliated to Wenzhou Medical College,Wenzhou 325027,China)
SHI Hai-fan(Department of Pediatrics,Yuying Children's Hospital Affiliated to Wenzhou Medical College,Wenzhou 325027,China)
WANG Hong-jiao(Department of Pediatrics,Yuying Children's Hospital Affiliated to Wenzhou Medical College,Wenzhou 325027,China)
CHEN Yi-ping(Department of Pediatrics,Yuying Children's Hospital Affiliated to Wenzhou Medical College,Wenzhou 325027,China)
LI Guang-qian(Department of Pediatrics,Yuying Children's Hospital Affiliated to Wenzhou Medical College,Wenzhou 325027,China)
Journal Title:
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
Issue:
Volume 2, Issue 05, 2009
DOI:
10.3760/cma.j.issn.1674-2397.2009.05.006
Key Word:
Japanese encephalitis; Interleukin-lbeta; Interleukin-1 receptor antagonist; Cerebrospinal fluid

Abstract: Objective To investigate the contents of IL-1β and IL-1ra in cerebrospinal fluid of children with Japanese encephalitis, and their clinical significance. Methods Enzyme linked immunosorbent assay (ELISA) was employed to detect IL-lβ and IL-lra contents in 50 children with Japanese encephalitis and 20 children without nervous system disease (controls). Results IL-1β contents in climax stage, convalescent stage and the controls were (49. 43±14. 59) , (24. 73±14. 50) and (8. 98± 1.26)μg/L (F = 79.88, P<0.01); IL-lra contents in climax stage, convalescent stage and the controls were (177. 39±60. 19), (78. 24±44. 63) and (21. 09±3. 10) μg/L (F = 91. 53, P <0. 01). There were significant differences on IL-lβ and IL-lra contents among children with mild, moderate and severe encephalitis (climax: F = 82.36 and 66.50, P<0.01; convalescence; F = 55. 17 and 79.50, P<0.01). IL-1β content was positively correlated with IL-lra in both climax and convalescent stages (climax; r = 0. 815, P < 0.01; convalescent; r= 0.728, P < 0.01). Conclusions IL-lβ and IL-lra contents in cerebrospinal fluid are significantly increased in children with Japanese encephalitis in climax stage, which are closely correlated with the disease severity. The two indicators may participate in the pathological process of brain damages with Japaness encephalitis.

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