Expression of Toll-like receptors in peripheral blood mononuclear cells and serum inflammatory cytokine in patients with liver failure

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Author:
LIN Xi(Department of Infectious Diseases,Taizhou Hospital of Zhejiang Province,Linhai 317000,China)
ZHU Jian-sheng(Department of Infectious Diseases,Taizhou Hospital of Zhejiang Province,Linhai 317000,China)
WANG Geng-ge(Department of Infectious Diseases,Taizhou Hospital of Zhejiang Province,Linhai 317000,China)
ZHU Min(Department of Infectious Diseases,Taizhou Hospital of Zhejiang Province,Linhai 317000,China)
CHEN Hua-zhong(Department of Infectious Diseases,Taizhou Hospital of Zhejiang Province,Linhai 317000,China)
ZHAO Hai-hong(Department of Infectious Diseases,Taizhou Hospital of Zhejiang Province,Linhai 317000,China)
Journal Title:
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
Issue:
Volume 2, Issue 04, 2009
DOI:
10.3760/cma.j.issn.1674-2397.2009.04.002
Key Word:
Liver failure;Toll-like receptors;Endotoxin;Tumor necrosis factor-α;Interleukin-6

Abstract: Objective To investigate the expression of Toll-like receptor 2 and 4 (TLR2 and TLR4) in peripheral blood mononuclear cells (PBMCs) and the serum inflammatory cytokine levels in patients with liver failure. Methods The expressions of TLR2 mRNA and TLR4 mRNA in PBMCs were detected by RT-PCR in 20 healthy controls, 20 chronic hepatitis B (CHB) patients, 18 liver failure patients in early stage and 14 in intermediate-end stage. The serum contents of endotoxin, TNF-α and IL-6 were detected by ELISA. Results Compared with the healthy controls, the expression of TLR2 mRNA and TLR4 mRNA, and the contents of endotoxin, TNF-α and IL-6 increased in CHB patients and liver failure patients ( both early stage and intermediate-end stage) ( F = 32.997, 37.476, 23. 951,57. 265 and 38. 403, P < 0.01 ). TLR2 mRNA expression in liver failure patients in intermediate-end stage was higher than that in the early stage, but that for TLR4 mRNA was lower than that in early stage. The expressions of TLR2 mRNA and TLR4 mRNA in PBMCs were significantly correlated with the contents of TNF-α and IL-6 ( r = 0. 917 and 0. 788, P < 0. 01 ). Conclusion The inflammation reaction mediated by TLR2 and TLR4 might participate in the pathogenesis of liver failure.

  • [1]Iwasaki A,Medzhitov R.Toll-like receptor control of the adaptive immune responses.Nat Immunol,2004,5(10):987-995.
  • [2]Liver Failure and Artifical Liver Group,Chinese Society of Infectious Disease,CMA and Severe Liver Diseases and Artificial liver Group,Chinese Society of Infectious Disease,CMA.Guideline for diagnosis and treatment of liver failure.Zhonghna Linchuang Ganranbing Zazhi,2008,1 (1):47-53.(in Chinese)中华医学会感染病学分会肝衰竭与人工肝学组,中华医学会肝病学分会重型肝病人工肝学组.肝衰竭诊疗指南.中华临床感染病杂志,2008,1(1):47-53.
  • [3]Parker LC,Prince LR,Sabroe I.Translational mini-review series on Toil-like receptors:networks regulated by Toll-like receptors mediate innate and adaptive immunity.Clin Exp Immunol,2007,147(2):199-207.
  • [4]Palsson-McDermott EM,O'Neill LA.Signal transduetion by the lipopolysaccharide receptor,Toll-like receptor-4.Immunology,2004,113(2):153-162.
  • [5]Takayashiki T,Yoshidome H,Kimuru F,et al.Increased expression of Toll-like receptor 4 enhances endotoxin-induced hepatic failure in partially hepatectomized mice.J Hepatol,2004,41 (4):621-628.
  • [6]Stadlbauer V,Mookerjee RP,Hodges S,et al.Effect of probiotic treatment on deranged neutrophil function and cytokine responses in patients with compensated alcoholic cirrhosis.J Hepatol,2008,48(6):945-951.
  • [7]Xing T,Li L,Cao H,et al.Altered immune function of monocytes in different stages of patients with acute on chronic liver failure.Clin and Exp Immunol,2007,147(1):184-188.
  • [8]Nomura F,Akashi S,Sakao Y,et al.Cutting edge:endotoxin tolerance in mouse peritoneal macrophages correlates with downregulation of surface Toll-like receptor 4 expression.J Immunol,2000,164(7):3476-3479.
  • [9]Ogawa H,Rafiee P,Heidemann J,et al.Mechanisms of endotoxin tolerance in human intestinal microvaseular endothelial cells.J Immunol,2003,170 (12):5956-5964.
  • [10]De Creus A,Abe M,Lan AH,et al.Low TLR4 expression by liver dendritic cells correlates with reduced capacity to activate allogeneic T cells in response to endotoxin.J Immunol,2005,174(4):2037-2045.
  • [11]Bieback K,Lien E,Klagge IM,et al.Hemagglntinin protein of wild-type measles virus activates toll-like receptor 2 signaling.J Virol,2002,76(17):8729-8736.
  • [12]Matsumura T,Hayashi H,Takii T,et al.TGF-beta downregulates IL-1 alpha-induced TLR2 expression in murine hepatocytes.J Leukoc Biol,2004,75(6):1056-1061.
  • [13]Hermoso MA,Matsuguchi T,Smoak K,et al.Glucocorticoids and tumor necrosis factor alpha cooperatively regulate Toll-like receptor 2 gene expression.Mol Cell Biol,2004,24(11):4743-4756.
  • [14]Musikacharoen T,Matsuguehi T,Kikuchi T,et al.NF-kappa B and STAT5 play important roles in the regulation of mouse Toll-like receptor 2 gene expression.J Immunol,2001,166 (7):4516-4524.
  • [15]Sato K,Ishikawa T,Okumura A,et al.Expression of Toll-like receptors in chrenie hepatitis C virus infection.J Gastreenterol Hepatol,2007,22(10):1627-1632.
  • [16]Iimuro Y,Seki E,Son G,et al.Role of innate immune response in liver regeneration.J Gastroenterol Hepatol,2007,22 (Suppl 1):S57-S58.
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