Multi-locus analysis of AD hybrid strains of Cryptococcus neoformans

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FENG Xiao-bo(Department of Dermatology,Xinhua Hospital,Shanghai Jiaotong University,School of Medicine,Shanghai 200092,China)
YAO Zhi-rong(Department of Dermatology,Xinhua Hospital,Shanghai Jiaotong University,School of Medicine,Shanghai 200092,China)
LING Bo(Department of Dermatology,Xinhua Hospital,Shanghai Jiaotong University,School of Medicine,Shanghai 200092,China)
REN Da-ming()
Journal Title:
Volume 2, Issue 03, 2009
Key Word:
Cryptococcus neoformans;Heterozygote;Mating type

Abstract: Objective To identify the AD hybrid strains and its hybrid types within Cryptococcus neoformans.Methods Difierent hybrid types of AD strains were analyzed by PCR 0f STE20 and MF genes within MAT locus and CIA4 and GPal genes out of MAT locus.The PCR-RFLP analysis of g6341 gene was also performed.Results The mating types of 18 AD strains were precisely identified by PCR of STE20 gene,whereas those of H strain were not identified.CL44 gene was better than the GPal gene in PCR identification of the AD hybrids.In the RFLP analysis of g6341 gene,AD strains were grouped into 2 distinct RFLP patterns based on the mating type on serotype A allele.The mating types of AD strains were not identified by the molecular analyses based on the CL44,GPal and g6341 genes.Conclusion It is necessary to use multi-locus analyses of genes within and out of the MAT locus in precise identification of the AD strains and their hybrid types of Cryptococcus neoformans.

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