Quantitative detecetion of HBsAg concentrations by chemiluminescent microparticle immunoassay and its relationships with anti-HBs,HBeAg and HBV DNA

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Author:
CHEN Wen-hu(Department of Clinical Laboratory,Zhejiang Canter Hosptial,Hangzhou 310022,China)
LOU Bin()
Journal Title:
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
Issue:
Volume 2, Issue 03, 2009
DOI:
10.3760/cma.j.issn.1674-2397.2009.03.004
Key Word:
Hepatitis B surface antigens;Chemiluminescent microparticle immunoassay;Quantitative detection

Abstract: Objective To investigate the clinical significance of quantitative detection of serum HBsAg.Methods Serum concentrations of HBsAg were detected by chemiluminescent microparticle immunoassay in 7612 hospitalized non-hepatitis patients.The distribution of HBsAg positive patients and the relationship of HBsAg concentration with serum anti.HBs.HBeAg and HBV DNA were analyzed.Results The HBsAg positive rate was 11.4%(870/7612)in this series.The serum HBsAg ranged from 0.08 U/mL to 125 000 U/mL with a median of 932.28 U/mL.No significant difference of HBsAg concentrations between male and female patients was observed(Z=-0.366,P>0.05).1.There were negative correlation of HBsAg concentration with the age of patients(r=-0.370,P<0.01),and positive correlations of HBsAg with HBeAg(r=0.654,P<0.01)and HBV DNA levels(r=0.765,P<0.01).Conclusion The quantitative determination of seFum HBsAg may be useful in estimating the hepatitis B viral replication and clearance.

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