The distribution of 16S rRNA methylase genes in KPC-producing Klebsiella pneumoniae strains

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Author:
LU Li-ying(State Key Laboratoryfor Diagnosis and Treatment of Infectious Diseases,the First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China)
ZHANG Wei-li(State Key Laboratoryfor Diagnosis and Treatment of Infectious Diseases,the First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China)
YANG Qing(State Key Laboratoryfor Diagnosis and Treatment of Infectious Diseases,the First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China)
ZHOU Hua(State Key Laboratoryfor Diagnosis and Treatment of Infectious Diseases,the First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China)
YU Yun-song(State Key Laboratoryfor Diagnosis and Treatment of Infectious Diseases,the First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China)
Journal Title:
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
Issue:
Volume 2, Issue 02, 2009
DOI:
10.3760/cma.j.issn.1674-2397.2009.02.003
Key Word:
Klebsiella pneumoniae;RNA methylase;Carbapenenase

Abstract: Objective To investigate the distribution of 16S rRNA methylase genes in Klebsiella pneumoniae strains producing Klebsiella pneumoniae ealbapenenase type 2(KPC-2).Methods A total of 37 Klebsiella pneumoniae isolates producing KPC-2 were collected.The minimal inhibitory concentrations (MICs)of these strains to amikacin,gentamyein and netilmicin were determinated by agal dilution method.Six 16S rRNA methylase genes(armA,rmtA,rmtB,rmtC,rmtD and npmA)were detected by PCR.Results The resistant rates to amikacin,gentamycin and netilmicin were 97.3%(MIC50≥1024μg/mL).Among those resistant strains,8 were armr/A positive,25 were rmtB positive,4 were both armA and rmtB positive.and no other 16S rRNA methylase genes were found.The total positive rate of 16S rRNA methylase genes was 78.4%(29/37).Conclusion 16S rRNA methylase genes armA and rmtB ale prevalent in Klebsiella pneumoniae strains producing KPC-2.

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