The genetic features of pan-drug resistant pseudomonas aeruginosa

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Author:
ZHANG Yu-yun(Department of Prevention and Health Promotion, Yantai Yuhuangding Hospital,Yantai 264000, China)
WU Jin-ying()
FAN Xiao-li(Department of Prevention and Health Promotion, Yantai Yuhuangding Hospital,Yantai 264000, China)
YAN Bo(Department of Prevention and Health Promotion, Yantai Yuhuangding Hospital,Yantai 264000, China)
Journal Title:
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
Issue:
Volume 1, Issue 05, 2008
DOI:
Key Word:
Pseudomonas aeruginosa;Pan-drng resistance;Drug resistance genes;Genetic markers

Abstract: Objective To investigate the genetic features of pan-drag resistant pseudomonas aeruginosa. Methods The susceptibilities to 14 antibacterial agents were detected in pseudomonas aerations by K-B paper diffusing method. A strain of pan-drug resistant pseudomonas aeruginosa was selected randomly, and was used to amplify genes for β-Lactam antibiotic resistance (TEM, SHV, CTX-M-1 group,CTX-M-2 group, CTX-M-9 group, OXA-1 group, OXA-2 group, OXA-10 group, PER, GES, VEB,CARB, LCR, BEL, DHA, IMP, VIM, SPM, GIM, SIM and oprD2), aminoglycosidase drug resistance genes (including aminoglycosides modification enzymes gent,16S rRNA methylase gene) and genetic markers of plasmid (traA and traF), integrons (tnpA, tnpU and merA), transposons Ⅰ (int Ⅰ 1 ) by PCR,and the sequences of above genes were analyzed. Results The pseudomonas aeruginosa was resistant to all 14 antibacterial agents, and the following genes were positive in PCR: TEM-1 and CARB-3 types of genes for β-Lactam antibiotic resistance (deletion of oprD2), aac (6')-Ⅱ and ant (2")-Ⅰ of aminoglycosidase resistance genes, transposons Ⅰ (int Ⅰ 1 ), and the merit of integrons. Conclusions Pseudomonas aeruginosa shows high resistance to most antibacterial agents, which should draw attention in clinic.

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