Characteristics of hepatic progenitor cells activation in liver tissues of patients with hepatitis B cirrhosis

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WU Nan(The Hepatology Institute of Peking University,Beijing 100044,China)
LIU Feng(The Hepatology Institute of Peking University,Beijing 100044,China)
MA Hui(The Hepatology Institute of Peking University,Beijing 100044,China)
ZHU Feng-xue()
LIU Zhi-da(The Hepatology Institute of Peking University,Beijing 100044,China)
FEI Ran(The Hepatology Institute of Peking University,Beijing 100044,China)
CHEN Hong-song(The Hepatology Institute of Peking University,Beijing 100044,China)
WANG Hao(The Hepatology Institute of Peking University,Beijing 100044,China)
WEI Lai(The Hepatology Institute of Peking University,Beijing 100044,China)
Journal Title:
Volume 1, Issue 03, 2008
Key Word:
Liver cirrhosis;Hepatic progenitor cells;Duetular reaction

Abstract: Objective To observe the characteristics of hepatic progenitor cells(HPCs)activation in liver tissues of patients with hepatitis B cirrhosis,and to investigate the relationship between the number of HPCs and HBV infection.Methods Cytokeratin 7(CK7)-was stained immunohistochemically in liver tissues of 16 patients with hepatitis B cirrhosis.HPCs and duetular reactions were quantitively analyzed.The expression of HBsAg and HBcAg were also detected to evaluate its relationship with HPCs activation.Results HPCs were extensively activated and marked duetular reactions can be observed in cirrhotic liver tissues.Tlle expression of HBsAg was positively correlated with HPCs activation.Conclusions HPCs are extensively activated in cirrhotic liver tissues,and HBV infection may facilitate its activation.

  • [1]Libbrecht L,Desmet V,Van Damme B,et al.Deep intralobular extension of human hepatic‘progenitor cells'correlates with parenchymal inflarmnation in chronic,viral hepatitis.can‘pmgenitor cells'migrate?.J Pathol,2000,192(3):373-378.
  • [2]Boskams TA,Theise ND,Balabaud C,et al.Nomenclature of the finer branches of the biliary tree:canals,ductules,and ductular reactions in human livers.Hepatology,2004,39(6):1739-1745.
  • [3]Lowes KN,Brennan BA,Yeoh GC,et al.Oval cell numbers in human chronic liver diseases are directly related to disease severity.Am J Pathol,1999,154(2):537-541.
  • [4]Roskams T,Yang SQ,Koteish A,et al.Oxidative stress and oval cell accumulation in mice and humans with alcoholic and nonalcoholic fatty liver disease.Am J Pathol,2003,163(4):1301-1311.
  • [5]Fotiadu A,Tfioufa V,Vrettou E,et al.Progenitor cell activation in chronic viral hepatitis.Liver Int,2004,24(3)1268-274.
  • [6]Eleazar JA,Memeo L,Jbeng JS,et al.Progenitor cell expansion:an important source of hepatocyte regeneration in chronic hepatitis.J Hepatol,2004,41(6)1983-991.
  • [7]Tan J,Hytirogiou P,Wieczorek R,et al.Immunohistochemical evidence for hepatic progenitor cells in liver diseases.Liver,2002,22(5):365-373.
  • [8]Hsia CC,Thorgeirsson SS,Tabor E.Expression of hepatitis B surface and core antigens and transforming growth factor-alpha in "oval cells"of the liver in patients with hepatocellular carcinoma.J Med Virol,1994,43(3):216-221.
  • [9]Knodell RG,Ishak KG,Black WC,et al.Formulation and application of a numerical scoring system for assessing histological activity in nsymptomatic chronic active hepatitis.Hepatology,1981,1(5):431-435.
  • [10]Ishak K,Baptism A,Bianchi L,et al.Histological grading and stasing of chronic hepatitis.J Hepatol,1995,22(6):696-699.
  • [11]Theise ND,Saxena R,Portmann BC,et al.The canals of Hering and hepatic stem ceils in humans.Hepatology,1999,30(6):1425-1433,
  • [12]Falkowski O,An HJ,hnus IA,et al.Regeneration of hepatocyte ‘buds'in cirrhosis from intrabiliary stem cells.J Hepatol,2003,39(3):357-364.
  • [13]Zhang W,Chen XP,Xiang S,et al.Hepatic progenitor cell activation and expansion in human liver cirrhosis.Zhanghua Ganzanghing Zazhi,2006,14(10):748-752.张伟,陈孝平,项帅,等.肝硬化中肝脏祖细胞的激活与扩增.中华肝脏病杂志,2006,14(10):748-752.
  • [14]Sun C,Jin XL,Xian JC.Oval cells in hepatitis B vires-positive and hepatitis C virus-positive liver cirrhosis:histological and ultrnstructural study.Histopathology,2006,48(5):546-555.
  • [15]Akhurst B,Matthews V,Husk K,et al.Differential lymphotoxinbeta and interferon ganana signaling during mouse liver regeneration induced by chronic and acute injury.Hepatology,2005,41(2):327-335.
  • [16]Brooling.IT.Campbell JS,Mitchell C,et al.Differential regulation of rodent hepatocyte and oval cell proliferation by interferon gamma.Hepatology,2005,41(4):906-915.
  • [17]Nguyen LN,Furuya MH,Wolfraim LA,et al.Transforming growth factor-beta differentially regulates oval cell and hepatocyte proliferation.Hepatology,2007,45(1):31-41.
  • [18]Knight B,Akhurst B,Matthews VB,et al.Attenuated liver progenitor(oval)cell and fibrogenic responses to the choline deficient,ethionine supplemented diet in the BALB/c inbred strain of mice.J Hepatol,2007,46(1):134-141.
  • [19]Davies SE,Pomnann BC,O'Grady JG,et al.Hepatic histological findings after transplantation for chronic hepatitis B virus infection,including a unique pattem of fibrosing cholestatic hepatitis.Hepatology,1991,13(1):150-157.
  • [20]Foo NC,Ahn BY,Ma X,et al.Cellular vaeuolization and apoptosis induced by hepatitis B virus large surface protein.Hepatology,2002,36(6):1400-1407.
  • [21]Meuleman P,Libbrecht L,Wieland S,et al.Immune suppression uncovers endogenous cytopathic effects of the hepatitis B virus.J Virol,2006,80(6)12797-2807.
  • [22]Wang GS,Wang MM,Xie QL,et al.Expression and clinical significance of HBsAg and HBcAg in hepatocytes in chronic hepatitis B.Zhonghua Gmgbing Zazhi,2004,12(5):287-289.王功遂,王曼曼,谢秋里,等.HBsAg,HBcAg在慢性乙型肝炎肝细胞内的表达及临床意义.中华肝脏病杂志,2004,12(5):287-289
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