HBV genotypes and gene mutations in patients with liver failure after withdrawal of lamivudine
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1674-2397.2008.02.005
- Key Word:
- 肝功能衰竭;拉米夫定;复发;基因型;突变;Liver failure;Lamivudine;Relapse;Genotype;Mutation
Abstract： Objective To investigate HBV genotypes and gene mutations in chrenic hepatitis B (CHB) patients with liver failure after lamivudine withdrawal. Methods Twenty four patients with relapsing CHB after lamivudine withdrawal were divided into liver failure group ( n = 12 ) and chronic hepatitis group ( n = 12 ). HBV DNA from these patients was amplified by PCR. The PCR products were cloned into PGEM-T vector and HBV DNA sequences were analyzed. Results In liver failure group, there were 6 sequences detected, in which 3 were of genotype B and 3 were of genotype C. In chronic hepatitis group, there were 9 sequences detected, in which 2 were of genotype B and 7 were of genotype C. Compared with the wild type HBV sequences, there were multiples mutations in S, P, C, X regions. Gene mutations in high conservative sequences of BCP and P regions were detected in liver failure patients after lamivudine withdrawal. Conclusions In HBV patients with liver failure after lamivudine withdrawal, half of them were of genotype B and the others were of genotype C. Some mutations in high conservative sequences of BCP and P regions may be related to the liver failure in these patients.
- [1]黄晶,高志良.乙型肝炎病毒基因型及其临床意义的研究[J].2002,(11).
- [2]李嘉,朱理珉,梁树人,等.乙型肝炎病毒核心区基因变异与细胞免疫[J].2003,(9).
- [3]Hiromi, Yatsuji,Chiemi, Noguchi,Nobuhiko, Hiraga,etc.Emergence of a novel lamivudine-resistant hepatitis B virus variant with a substitution outside the YMDD motif.[J].2006,50(11).
- [4]Kuiper M,Warner N,Locarnini S,etc.The L80I substitution in the reverse transcriptase domain of the hepatitis B virus polymerase is associated with lamivudine resistance and enhanced viral replication in vitro.[J].2007,51(7).
- [5]郭亚乒,冯筱榕,戴炜,等.T_（1762）A_（1762）变异对乙型肝炎病毒C启动子活性影响[J].1999,(1).
- [6]Yotsuyanagi H,Hino K,Iino S,etc.Precore and core promoter mutations, hepatitis B virus DNA levels and progressive liver injury in chronic hepatitis B.[J].2002,37(3).
- [7]Niel C,Bottecchia M,Ikuta N,etc.Lamivudine resistance and other mutations in the polymerase and surface antigen genes of hepatitis B virus associated with a fatal hepatic failure case.[J].2008,23(1).
- [8]Du WB,Pan XP,Li LJ,etc.Differences of YMDD mutational patterns, precore/core promoter mutations, serum HBV DNA levels in lamivudine-resistant hepatitis B genotypes B and C.[J].2007,14(11).
- [9]Dhillon AP,Lindh M,Hannoun C,etc.Core promoter mutations and genotypes in relation to viral replication and liver damage in East Asian hepatitis B virus carriers.[J].1999,179(4).
- [10]Dutronc H,Schvoerer E,Dubois F,etc.Hepatitis B exacerbation with a precore mutant virus following withdrawal of lamivudine in a human immunodeficiency virus-infected patient.[J].2000,41(2).