Expression of signal transduction molecule STAT-1 in patients with chronic hepatitis B after interferon-alpha therapy

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Author:
WANG Lu-wen(Department of Infectious Diseases, State Key Laboratory of Virology, Renmin Hospital of Wuhan University, Wuhan 430060, China)
GONG Zuo-jiong(Department of Infectious Diseases, State Key Laboratory of Virology, Renmin Hospital of Wuhan University, Wuhan 430060, China)
Journal Title:
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
Issue:
Volume 1, Issue 02, 2008
DOI:
Key Word:
Janus kinase;Signal transduction;Activating transcription factor;Chronic hepatitis B;Interferon

Abstract: Objective To investigate the expression of signal transduction molecule STAT-1 in patients with chronic hepatitis B (CHB) who received interferon-alpha (IFNα) treatment with different responses. Methods A total of 13 CHB patients received IFNα treatment, among who 5 demonstrated complete viral response ( Group A) and 8 showed no response ( Group B). All patients underwent liver biopsy before and after the therapy. STAT-1 mRNA of liver tissue was semi-quantified by RT-PCR and levels of STAT-1 protein were detected by Western-blot. Results Absorbance values (A) of STAT-1 mRNA in group A and B were (1. 18±0.06) and (0.21±0.04) respectively (t =35.27, P<0. 01). The levels of STAT-1 protein of liver tissue in group A were also significantly higher than those in group B. Conclusion IFNα resistance may be closely related to the down-regulation of STAT-1.

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