Hepatitis B virus facilitates the expression of MMP-2 and TIMP-1 in hepatic stellate cells

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Author:
HA Ming-hao(The Hepatology Institute of Peking University,Beijing 100044,China)
RAO Hui-ying(The Hepatology Institute of Peking University,Beijing 100044,China)
LIU Feng(The Hepatology Institute of Peking University,Beijing 100044,China)
FEI Ran(The Hepatology Institute of Peking University,Beijing 100044,China)
CONG Xu(The Hepatology Institute of Peking University,Beijing 100044,China)
CHEN Hong-song(The Hepatology Institute of Peking University,Beijing 100044,China)
WEI Lai(The Hepatology Institute of Peking University,Beijing 100044,China)
Journal Title:
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
Issue:
Volume 1, Issue 01, 2008
DOI:
Key Word:
Hepatitis B virus;Liver fibrosis;Hepatic stellate cell;Matrix metalloproteinase-2;Tissue inhibitor of metalloproteinase-1;Co-culture

Abstract: Objective To investigate the effect of HBV on the expression of fibrosis-related factors in hepatic stellate cells(HSC)and its relation with liver fibrosis.Methods HSCs were co-cultured with HepG2 or HepG2.2.15 in vitro and HSCs cultured alone served as the control.The mRNA expression of matrix metalloproteinase(MMP)-2 and tissue inhibitor of metalloproteinase(TIMP)-1 was detected by realtime PCR.The protein expression of MMP-2 and TIMp-1 was detected by Western-blot.Results Compared with the control and the HSCs co-cultured with HepG2,the expression of MMP-2 and TIMP-1 mRNA in HSCs co-cultured with HepG2.2.15 was increased remarkably and the most significant difference was found at 72 h(F=11.91,23.13;P=0.008,0.001);the expression of MMP-2 and TIMP-1 protein in HSCs co-cuhured with HepG2.2.15 was also increased remarkably and the most significant difference was found at 72 h(F=20.70,6.54;P=0.002,0.003)too.Conclusion The expression of fibrosis-related factors in HSCs increased significantly after co-cultured with HepG2.2.15,which suggests that HBV could promote liver fibrosis.

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