Study of Ag-containing on casting cobalt chromium alloy on the surface structure and the cell toxicity in vitro

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Author:
ZHAO Min(Department of Prosthodontics, College of Stomatology, Hebei United University, Tangshan Heibei 063000,China)
LIANG Rui-ying(Department of Prosthodontics, College of Stomatology, Hebei United University, Tangshan Heibei 063000,China)
MENG He(Department of Prosthodontics, College of Stomatology, Hebei United University, Tangshan Heibei 063000,China)
XU Yan-li(Department of Prosthodontics, College of Stomatology, Hebei United University, Tangshan Heibei 063000,China)
LI Jing-dong(Department of Prosthodontics, College of Stomatology, Hebei United University, Tangshan Heibei 063000,China)
WU Wen-hui(Department of Prosthodontics, College of Stomatology, Hebei United University, Tangshan Heibei 063000,China)
Journal Title:
Chinese Journal of Stomatology
Issue:
Volume 47, Issue 10, 2012
DOI:
10.3760/cma.j.issn.1002-0098.2012.10.012
Key Word:
Chromium alloys; Cytotoxicity tests, immunologic; Fibroblasts; Cell cycle

Abstract: Objective To detect cobalt chromium alloy antimicrobial coating silver of the surface structure and the cell toxicity in order to provide a theoretical basis for clinical application.Methods Plasma spraying technique was adopted to prepare cobalt chromium alloy antimicrobial coating silver. Scanning electron microscopy,energy dispersive analysis and X-ray diffraction analysis were used to evaluate the surface properties.The methyl thiazolyl tetrazolium and flow cytometry method was adopted to test the L929 cell proliferation and the influence of the cell cycle. Results The surface of the coating was uniform and compact,combined perfectly with substrate material. The content of the surface was mainly Ag,Cr and a small amount of Ag2O,Cr2O3.After cobalt chromium alloy was cultured in leach liquor for 1,2 and 3 days,the statistical result showed that there was no significant different between the three groups.The cytotoxic level of negative control group was level 0 at each time point and that of other groups was level 1 at each time point.There was no significant difference between cobalt chromium alloy and cobalt chromium alloy antimicrobial coating silver in cell toxicity(P > 0.05).There was no statistical significance of the influence on cell cycle between cobalt chromium alloy with Ag coating [ the G2' s rate of cell cycle was ( 8.23 ±0.39 ) % ] and cobalt chromium alloy group [ the G2's rate of cell cycle was ( 8.70 ± 0.46 ) % ] ( P > 0.05 ).Conclusions The surface of the coating was stable and there was no significant difference between cobalt chromium alloy widely used in clinic and cobalt chromium alloy with Ag coating of the influence on proliferation of L929 cell and cell cycle,the cell compatibility of cobalt chromium with Ag coating is well.

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