Abstract： Objective To investigate the influnce of receptor activity modifying protein 1 ( RAMP-1 ) overexpression on enhancing effect of calcitonin gene-related peptide on MG-63 cells proliferation. Methods Cultured MG-63 osteoblasts in exponential phase of growth were randomly divided into three groups:RAMP-1 overexpression group,empty vector control group and negative control group. RAMP-1 eukaryotic expression vector was constructed and stalely transfected into MG-63 cells. Realtime-polymerase chain reaction,Western blotting and immunofluroescence were used respectively to detect the expression of calcitonin receptor-like receptor( CRLR ) in the cells and its distribution on cell membrane.The status of proliferation was detected respectively at 0,24,48,72,96 h by cell counting kit-8( CCK-8 ) and cells were collected to analyze their cycle respectively at 0,8,16,24 h by flow cytometry.Results CRLR protein and mRNA expression levels of MG-63 cells in RAMP-1 overexpression group were significantly higher than the other two groups (P＜0.05).The A value of RAMP-1 overexpression group at 24,48,72,96 h were 0.628 ±0.175,0.896 ±0.592,1.055 ±0.004,1.179 ±0.618,respectively,which were significantly higher than that of the other two groups( P ＜0.05).The difference was most pronounced at 72 h.S-phase fraction of RAMP-1 overexpression group was ( 1.25 ± 0.13 ) ％,( 68.79 ± 0.56 ) ％,( 64.49 ± 1.59 ) ％,( 57.82 ± 0.75 ) ％,respectively,which were significantly higher than the other two groups ( P ＜ 0.05 ). The difference was most pronounced at 8 h.Conclusions RAMP-1 overexpression can promote CRLR distribution on MG-63 cell membrane and enhance CGRP's promotion effect on MG-63 cell proliferation.