Construction of tissue engineering bone with bone marrow stromal cell sheets
- Author:
- No author available
- Journal Title:
- Chinese Journal of Stomatology
- Issue:
- 12
- DOI:
- 10.3760/cma.j.issn.1002-0098.2011.12.012
- Key Word:
- 骨髓;骨髓细胞;组织工程骨;Bone marrow;Bone marrow cells;Tissue-engineered bone
Abstract： Objective To construct tissue engineering bone with bone marrow stromal cell(BMSC)sheets of dogs.Methods BMSC were derived from dog bone marrow and cell sheets were prepared with temperature-responsive dishes after the cells were induced by osteogenesis.Allogeneic dogs decalcification bone matrixes(DBM) were prepared.Sixteen dogs were divided into 4 groups.The MSC cell sheets-rhBMP2-BMSC-DBM were implanted under the left latissimus dorsi myofascial as the experimental side; while the thBMP-2-BMSC-DBM were implanted in the right side as the control.Ectopic bone formation in vivo was evaluated by histological examination 4,8,12,16 weeks after operation.Results The osteogenesis in the experimental group was better than that in the control group.New bone area in the experimental side was larger than that in the control group,and the difference was significant ( P ＜ 0.05 ).After 16 weeks,lamellar bone was connected into a film in the experimental group.Haversian system and red bone marrow could be seen.Conclusions BMSC cell sheets could promote the bone formation of functional tissueengineered bone.
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