Effect of longterm lower Porphyromonas gingivalis infection on the progression of atherosclerosis in apolipoprotein E-knocked out mice
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1002-0098.2011.02.003
- Key Word:
- 紫单胞菌,龈;动脉粥样硬化;小鼠,基因敲除;Porphyromonas gingivalis;Atherosclerosis;Mice,knockout
Abstract： Objective To assess the effect of longterm and lower oral inoculation with Porphyromonas gingivalis (Pg) on the progression of atherosclerosis in apolipoprotein E-knocked out (ApoE -/-) mice. Methods Six-week-old male ApoE -/- mice were inoculated orally with 0. 1 ml live Pg(1013/L) or bouillon culture-medium quintic per week for 15 consecutive weeks, altogether 75 times of inoculations. The lesion area of atherosclerosis in the aortic tree was measured by en face quantification by red oil O staining method. The atherosclerotic lesion was examined by histopathology. The levels of total cholesterol and triglycerides were compared. Results At 22 weeks after inoculation, the mean atherosclerotic lesion area in inoculated mice was (98 363.68 ± 12 043.00) μm2 ,which was significantly greater than that in noninoculated mice, which was (62 985.06 ± 7419. 64) μm2 (P = 0. 035).Conclusions Longterm lower oral inoculation of Pg can accelerate the progression of atherosclerosis in apolipoprotein E-knocked out mice.
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