You Position: Home > Paper

Influence of surface roughness of titanium implant on core binding factor alpha 1 subunit of osteoblasts

( views:340, downloads:76 )
Author:
No author available
Journal Title:
CHINESE JOURNAL OF STOMATOLOGY
Issue:
8
DOI:
10.3760/cma.j.issn.1002-0098.2010.08.006
Key Word:
钛;牙种植;核心结合因子α1亚基;表面粗糙度;Titanium;Dental implantation;Core binding factor alpha 1 subunit;Surface roughness

Abstract: Objective To determine whether the roughness of titanium implant can influence its osseointegration by affecting the growth, alkaline phosphatase (ALP) production and expression of core binding factor alpha 1 subunit (Cbfα1) of osteoblasts. Methods Totally 48 titanium disc specimens,15 mm in diameter and 2 mm in thickness, were included in the study and divided equally into 4 groups with 12 specimens in each group. Specimens were coarsened by sandblasting with carborundum granula and acid etching with mixing liquid of hydrochloric acid and sulphuric acid under diverse conditions. In the four groups, three were treated with different surface roughness: micro-roughness [ (1.00 ± 0. 20 ) μm ],midrange roughness [ ( 1.67 ± 0. 08 ) μm ] and severe roughness [ ( 2. 40 ± 0. 20) μm ], while the group untreated with surface roughness [ (0. 12 ± 0. 03 ) μm ] was set as control Scanning electron microscope,acridine orange fluorescence staining and coomassie brilliant blue staining were used to observe morphology and growth of osteoblasts incubating on these specimens. Enzyme linked immunosorbent assay(ELISA) and fluorescence quantitative real-time polymerase chain reaction were used to evaluate ALP production and gene expression of Cbfαl of osteobalsts among different groups. Results Growth, ALP production and CbfαlmRNA expression of osteoblasts in experimental groups were higher than those in control group( P <0. 05 ).Significant differences of these data were also found among three experimental groups ( P < 0. 05 ). Midrange roughness group showed the highest level of gene expression of Cbfαl mRNA, which was 0. 93 ± 0. 03.While that in the micro-roughness group (0. 50 ± 0. 03 ) came second, and the severe roughness group had the lowest data, which was 0. 37 ± 0. 07. Conclusions Results indicated that rough surface was more suitable for the adherence and propagation of the osteoblasts than smooth one did. Surface with roughness of 1~2 μm may be a better choice for osseointegration between osteoblasts and dental implants than others are.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn