You Position: Home > Paper

A differential expression proteomic study of human periodontal ligament cell during osteogenic differentiation

( views:226, downloads:8 )
Author:
No author available
Journal Title:
CHINESE JOURNAL OF STOMATOLOGY
Issue:
4
DOI:
10.3760/cma.j.issn.1002-0098.2009.04.005
Key Word:
蛋白质组学;电泳;凝胶;双向;人牙周韧带细胞;成骨分化;Proteomics;Electrophoresis,gel,two-dimensional;Human periodontal ligament cell;Osteogenic differentiation

Abstract: Objective To obtain regulating proteins during human periodontal ligament cell (hPDLC) nstengenic differentiation and investigate its underlying molecular mechanism. Methods Two-dimension difference in gel electrophoresis (2D-DIGE) coupled with matrix-assissted laser adsorption ionization-time of flight mass spectrometry ( MALDI-TOF-MS ) analysis was used to identify regulating proteins during hPDLC osteogenic differentiation. Results A differently expressed protein profile was obtained 7 days after osteogenic induction, and 61 protein spots with significant differences, 29 protein spots were identified by MALDI-TOF-MS, including cytoskeleton, cell membrane-bounding, nuclear regulation,matrix synthesis, and metabolic enzymes and signal tnmsduction and other proteins. A functional class of cyteskeleton proteins showed co-regulation, these proteins were intimately involved in the reorganization of the cytoskeleton, cytokinesis and migration during PDLC differentiation. Conclusions A database of differently displayed proteins during hPDLC osteogenic differentiation was established, which may be helpful to understand and further study molecular mechanism of PDLC osteogenic differentiation.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn