Expression of chemokine receptor-7 in oral squamous cell carcinoma and its relation to cervical lymph node metastasis
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 10
- DOI:
- 10.3321/j.issn:1002-0098.2008.10.005
- Key Word:
- 癌,鳞状细胞;肿瘤转移;受体,趋化因子;Carcinoma,squamous cell;Neoplasm metastasis;Receptors,chemokine
Abstract： Objective To investigate the expression of chemokine receptor CCR7 in oral squamous cell carcinoma (OSCC) and its possible role in directional neck lymph node metastasis. Methods The expression of chemokine receptor CCR7 was examined in 85 cases of OSCC,normal oral mucosa,and TcaS113 and adenoid cystic carcinoma (ACC-M) cell lines using immunohistochemistry,RT-PCR and Western blotting.The relationship between the expression and clinicopathological factors of OSCC was analyzed.After treatment with or without CCR7 antibody,in vitro adhesion assay was performed to compare the adhesion of Tea8113 and ACC-M cells to lymph nodes. Results CCR7 was expressed in 66% (56/85)of OSCC cases,and there was a significant difference in CCR7 expression between metastasis group (31/39,79%) and non-metastasis group (25/46,54%).RT-PCR and Western blotting also confirmed the presence of positive CCR7 expression in OSCC. Tea8113 cells expressing CCR7 showed stronger ability to adhere to lymph nodes as compared to CCR7-negative ACC-M cells and could be actively inhibited by CCR7 antibody.Conclusions CCR7 may play an important role in the development and lymph node metastasis of oral squamous cell carcinoma.
- [1]王海丞,李翠英.纤维连接蛋白EDA片段在肿瘤生长、转移过程中的功能研究进展[J].2007,(4).
- [2]GarthL.Nicolson.Paracrine and autocrine growth mechanisms in tumor metastasis to specific sites with particular emphasis on brain and lung metastasis[J].1993,12(3).
- [3]R, F?rster,L, Ohl,G, Henning.Lessons learned from lymphocytes: CC chemokine receptor-7 involved in lymphogenic metastasis of melanoma.[J].2001,93(21).
- [4]Iwao, Takanami.Overexpression of CCR7 mRNA in nonsmall cell lung cancer: correlation with lymph node metastasis.[J].2003,105(2).
- [5]A, Müller,B, Homey,H, Soto,etc.Involvement of chemokine receptors in breast cancer metastasis.[J].2001,410(6824).
- [6]Wilson JL,Balkwill F,Levinson NR,etc.The chemokine network in cancer - much more than directing cell movement[J].2004,48(5).
- [7]Takashi, Murakami,Wusi, Maki,Adela R, Cardones,etc.Expression of CXC chemokine receptor-4 enhances the pulmonary metastatic potential of murine B16 melanoma cells.[J].2002,62(24).
- [8]Cyster JG,Williams LT,Tam C,etc.A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes.[J].1998,95(1).
- [9]Yongzeng, Ding,Yutaka, Shimada,Masato, Maeda,etc.Association of CC chemokine receptor 7 with lymph node metastasis of esophageal squamous cell carcinoma.[J].2003,9(9).
- [10]R, Stauder,S, Hamader,B, Fasching,etc.Adhesion to high endothelial venules: a model for dissemination mechanisms in non-Hodgkin's lymphoma.[J].1993,82(1).
- [11]Saeki H,Moore AM,Hwang ST,etc.Cutting edge: secondary lymphoid-tissue chemokine (SLC) and CC chemokine receptor 7 (CCR7) participate in the emigration pathway of mature dendritic cells from the skin to regional lymph nodes.[J].1999,162(5).
- [12]Reinhold Förster,Andreas Schubel,Dagmar Breitfeld,etc.CCR7 Coordinates the Primary Immune Response by Establishing Functional Microenvironments in Secondary Lymphoid Organs[J].1999,99(1).
- [13]J J, Campbell,E C, Butcher.Chemokines in tissue-specific and microenvironment-specific lymphocyte homing.[J].2000,12(3).
- [14]A, Peled,I, Petit,O, Kollet,etc.Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4.[J].1999,283(5403).