Monocyte chemoattractant protein-1 regulations of human gingival fibroblasts by Porphyromonas gingivalis with different fimA genotypes
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1002-0098.2008.05.004
- Key Word:
- 紫单胞菌,龈;基因型;成纤维细胞;趋化因子CCL2;Porhyromonas gingivalis;Genotype;Fibroblasts;Chemokine CCL2
Abstract： Objective To investigate the mechanism of monocyte chemoattractant protein-1 (MCP-1)regulations of human gingival fibroblasts(HGF)by challenge of Porphyromonas gingivalis(Pg)with different fimA genotypes.Methods Pg ATCC 33277(type Ⅰ),WCSP115(type Ⅱ),WCSP1.5(type Ⅲ),W83(type Ⅳ)were assessed for their inductions of MCP-1 expression in HGF.MCP-1 mRNA levels of HGF were determined by real-time RT-PCR and MCP-1 protein levels in culture supernatant by ELISA at different time intervals(1h,3h,6h and 12h)following continuous co-culture of bacteria with HGF.Results MCP-1 mRNA and protein levels were both up-regulated when HGF co-cultured with different Pg fimA genotypes.Type Ⅱ was stronger than other fimA genotypes,HGF expressed significantly great amount of MCP-1 mRNA[(25.75±3.12)-(326.69±35.35)]and protein[(178.20±46.20)-(443.46±82.19)ng/L]for different time periods;While Type Ⅲ was weaker than other fimA genotypes,and the level of MCP-1 mRNA was[(4.16±0.82)-(94.17±18.56)]and protein[(86.95±23.90)-(264.01±28.59)ng/L](P<0.05).Conclusions fimA genotypes of Pg are related with the inductions of MCP-1,which might indicate fimA genotype is associated with pathogenesis of Pg.
- [1]Ichiro, Nakagawa,Hiroaki, Inaba,Taihei, Yamamura,etc.Invasion of epithelial cells and proteolysis of cellular focal adhesion components by distinct types of Porphyromonas gingivalis fimbriae.[J].2006,74(7).
- [2]T, Hiraoka,Y, Izumi,T, Sueda.Immunochemical detection of CD14 on human gingival fibroblasts in vitro.[J].1998,13(4).
- [3]Zhou XD,Zhao L,Meng S,etc.Prevalence of fimA genotypes of Porphyromonas gingivalis and periodontal health status in Chinese adults.[J].2007,42(6).
- [4]Ichiro, Nakagawa,Atsuo, Amano,Masae, Kuboniwa,etc.Functional differences among FimA variants of Porphyromonas gingivalis and their effects on adhesion to and invasion of human epithelial cells.[J].2002,70(1).
- [5]S, Dogan,F, Gunzer,H, Guenay,etc.Infection of primary human gingival fibroblasts by Porphyromonas gingivalis and Prevotella intermedia.[J].2000,4(1).
- [6]Gibson FC,Takahashi Y,Genco CA,etc.Fimbria-dependent activation of pro-inflammatory molecules in Porphyromonas gingivalis infected human aortic endothelial cells[J].2006,8(5).
- [7]Akiyama S,Nakagawa I,Kuboniwa M,etc.Prevalence of specific genotypes of Porphyromonas gingivalis fimA and periodontal health status.[J].2000,79(9).
- [8]Nakagawa I,Nakano K,Kuboniwa M,etc.Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype[J].2007,9(3).
- [9]Hamdy, Nassar,Hsin-Hua, Chou,Mary, Khlgatian,etc.Role for fimbriae and lysine-specific cysteine proteinase gingipain K in expression of interleukin-8 and monocyte chemoattractant protein in Porphyromonas gingivalis-infected endothelial cells.[J].2002,70(1).