You Position: Home > Paper

Effect of fibrinogen on the secretion of interleukin-1β and-8 by polymorphonuclear leukocytes

( views:211, downloads:20 )
Author:
No author available
Journal Title:
CHINESE JOURNAL OF STOMATOLOGY
Issue:
1
DOI:
10.3321/j.issn:1002-0098.2008.01.004
Key Word:
纤维蛋白原;中性白细胞;牙周炎;Fibrinogen;Neutrophils;Periodontitis

Abstract: Objective To investigate the effect of one of the acute-phase proteins,fibrinogen,on the release of IL-1β and-8 by human peripheral polymorphonuclear leukocytes (PMN)and the possible role of fibrinogen during the destruction of periodontium. Methods Peripheral PMN were isolated by discontinuous density gradient centrifuging technique. The freshly isolated PMN were suspended in Hank's balaneed saline solution(1×109/L)supplemented with 0.5% BSA and 0.1% glucose.The levels of I-1β and-8 in the supernatants produced by cultured cells upon the addition of human fibrinogen at different concentrations were measured by ELISA technique. Results Incubated with human fibrinogen at 2 g/L or 10 g/L for different time periods,human peripheral PMN released significantly greater amount of IL-1β [(10.41±0.37)-(35.86±0.30)ng/L or(22.81 ± 0.45)-(57.77±2.08)ng/L]and IL-8[(93.90 ±13.95)-(2045.66±53.03)ng/L or(115.02±10.61)-(3858.69 ± 25.65)ng/L]than PMN without the stimulation offibrinogen(IL-1β,P<0.001,and IL-8,P≤0.016).The higher concentration of fibrinogen or the longer treatment time,the higher levels of IL-1β and -8 were released by PMN(P<0.001).Conclusions Fibrinogen induced the secretion of pro-inflammatory cytokines IL-1β and-8 by PMN and may be involved in magnification of the inflammatory response of periodontium and bone resorption.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn