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Early-response of the condylar chondrocyte under cyclic uniaxial compressive stress
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:1002-0098.2007.09.005
- Key Word:
- 下颌骨髁状突;软骨细胞;压力;蛋白质组学
Abstract： 目的 探讨体外周期性单轴压应力对大鼠髁突软骨细胞蛋白质表达的早期影响,为阐明髁突软骨细胞的力学应答反应机制奠定基础.方法 对第三代大鼠髁突软骨细胞进行周期性单轴压应力加载,分为2000 μstrain加力组和对照组、4000 μstrain加力组和对照组,加力时间为60 min;采用双向凝胶电泳技术和质谱鉴定分析软骨细胞蛋白质表达的变化.结果 与对照组相比,2000 μstrain加力组细胞蛋白质表达变化无统计学意义(P＞0.05);4000 μstrain加力组细胞蛋白质表达改变,3个新蛋白点出现,5个蛋白点消失,22个蛋白点表达下调,7个蛋白点表达升高(P＜0.05).质谱鉴定其中8个差异蛋白点分别为细胞骨架相关蛋白(丙种肌动蛋白和中间丝波形蛋白)、糖代谢相关蛋白(烯醇酶α和应激70糖调控蛋白)、信号传导相关蛋白(Raf激酶抑制蛋白),以及几种蛋白复合物.结论 在体外4000 μstrain周期性单轴压应力刺激下,大鼠髁突软骨细胞的蛋白表达谱明显改变,这些差异蛋白可能参与早期的力学应答反应.
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