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Transmission way of oral Streptococcus mutans in children
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1002-0098.2006.05.005
- Key Word:
- 链球菌,变异;基因型;聚合酶链反应;多态性,限制性片段长度
Abstract： 目的通过对婴儿院儿童及其母亲之间、全托儿童之间口腔中变形链球菌(简称变链菌)传播方式的研究,为儿童龋病预防提供新的思路.方法选取四川省实验婴儿院3～4岁的20名非全托儿童及其母亲和24名全托儿童作为研究对象,无菌牙签收集其菌斑样本,MSB培养基中培养48 h,每种表型菌落挑取一个代表株在TPY平板上次代纯培养,经形态学和微量生化鉴定后对分离的变链菌株行AP-PCR扩增,对不同个体出现相似基因型的变链菌株进行分析.结果44名儿童中,65.9%的儿童口腔内检出变链菌,20对母子中有10对均检出变链菌,44名儿童及20名母亲口腔中共分离出98株变链菌;均有变链菌检出的10对母子口腔中分辨出32个不同的基因型,其中7对母子有相似基因型出现;24名全托儿童口腔中共分辨出29个不同的基因型,有2种基因型在13名全托儿童口腔中有重复检出.结论变链菌在婴儿院儿童中存在水平传播及垂直传播两种传播方式.
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