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Effects of tea polyphenols on cell proliferation and hTERT of human Tca8113 cell lines
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1002-0098.2005.06.004
- Key Word:
- 癌,鳞状细胞;肿瘤细胞,培养的;茶多酚
Abstract： 目的观察茶多酚对人舌鳞状细胞癌Tca8113细胞增殖和端粒酶催化亚基(hTERT)的影响.方法实验分0.025、0.050、0.100、0.200 g/L茶多酚组和空白培养基组.采用噻唑蓝比色法检测茶多酚对Tca8113细胞增殖的影响,逆转录PCR法检测hTERT基因表达,Western-blot法测定细胞中hTERT蛋白表达量.所有数据采用ANOVA单因素方差分析和Student-Newman-Keuls检验进行统计学检验.结果茶多酚能明显抑制Tca8113细胞增殖,并存在时间和浓度依赖性.72 h时,0.200、0.100、0.050和0.025 g/L茶多酚组细胞增殖抑制率分别为69.75%±3.24%、63.17%±3.19%、50.35%±4.21%和34.75%±3.71%.72 h时,0.100、0.050 g/L组hTERT mRNA和蛋白表达均低于空白对照组(P<0.05).结论茶多酚能够抑制人舌鳞状细胞癌Tca8113细胞株的增殖及端粒酶催化亚基的转录和表达,抑制端粒酶催化亚基可能是茶多酚抗舌癌的机制之一.
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