You Position: Home > Paper
Application of alginate three-dimensional culture system for in vitro culture o f mandibular condylar chondrocytes from human osteoarthritic temporomandibular joint
( views:246, downloads:4 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:1002-0098.2002.04.003
- Key Word:
- 颞下颌关节;下颌骨髁状突;软骨细胞
Abstract： 目的将藻酸盐凝胶三维培养体系应用于颞下颌关节骨关节病(osteoar thritis of temporomandibular joint, TMJOA)髁突软骨细胞的体外培养.方法利用机械与酶消化的方法从TMJOA患者手术切除患疾髁突软骨中获得髁突软骨细胞, 部分细胞在二维贴壁培养条件下体外培养1周;部分细胞在高密度条件下转入藻酸盐凝胶培 养介质,进行三维培养4周;分别对贴壁条件下培养细胞和藻酸盐细胞凝胶微球石蜡包埋切片进行Ⅱ型胶原和软骨特异性蛋白多糖的免疫组化鉴定.结果从骨关节 病髁突软骨组织中收获并行体外培养成活的细胞,鉴定为软骨细胞;体外培养的藻酸盐凝胶 三维培养体系中的髁突软骨细胞生长状态良好;培养4周后细胞保持了良好的分化表型.结论成功地体外培养成活了人TMJOA髁突软骨细胞;成功地将藻酸盐凝胶三 维培养体系应用于人TMJOA髁突软骨细胞体外培养,在该体系中,软骨细胞生长状态与功能蛋白分泌能力良好.
- [1]常嘉,马绪臣,孙开华,等.颞下颌关节骨关节病髁状突软骨细胞体外培养及细胞生物学特性研究[J].2001,(6).
- [2]常嘉,马绪臣,魏明洁,等.兔髁状突软骨细胞藻酸盐凝胶三维培养体系的建立[J].2002,(2).
- [3]曲绵域,陈慧敏,刘剑刚,等.软骨细胞培养过程中细胞反分化的研究[J].1992,(4).
- [4]P D, Benya,J D, Shaffer.Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels.[J].1982,30(1).
- [5]van Osch-GJ,Verhaar JA,Verwoerd Verhoef-HL,etc.Tissue-engineered cartilage using serially passaged articular chondrocytes. Chondrocytes in alginate, combined in vivo with a synthetic (E210) or biologic biodegradable carrier (DBM).[J].2000,21(6).
- [6]van-der-Veen SW,van-Osch GJ,Verwoerd-Verhoef HL.In vitro redifferentiation of culture-expanded rabbit and human auricular chondrocytes for cartilage reconstruction.[J].2001,107(2).
- [7]姚向前,马绪臣,张震康.人胚髁状突软骨细胞的分离、培养和生物学性状[J].1997,(4).
- [8]J F, Guo,G W, Jourdian,D K, MacCallum.Culture and growth characteristics of chondrocytes encapsulated in alginate beads.[J].1989,19(2-4).