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Influence of HPV16 on expression of Rb, p16 and cyclin D1 in oral epithelial cell
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1002-0098.2002.02.002
- Key Word:
- 乳头状瘤病毒,人;上皮细胞;永生化
Abstract： 目的探讨16型人类乳头状瘤病毒(human papillomaviruses,HPV)对口腔上皮细胞Rb、p16、Cyclin D1表达的影响,以明确HPV16在口腔上皮细胞癌变过程中的作用机制.方法 Western印迹检测人永生化口腔上皮细胞HIOEC中HPV16 E6、E7、Rb、p16、Cyclin D1蛋白表达,免疫沉淀-Western印迹偶联检测HIOEC中HPV16E7-Rb复合物形成情况.结果 HIOEC表达HPV16 E6、E7蛋白;HIOEC出现去磷酸化Rb堆积,HPV16 E7和去磷酸化Rb形成复合物;p16、Cyclin D1蛋白表达无改变.结论 HPV16 E7在HPV16诱导的口腔上皮细胞永生化过程中起了重要作用.
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