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Effect of SP on intracellular free calcium concentration in cultured osteoblast
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1002-0098.2001.06.003
- Key Word:
- 生长物质;成骨细胞;细胞培养
Abstract： 目的观察P物质对体外培养成骨细胞内游离钙浓度的影响.方法采用细胞培养技术,以钙离子敏感荧光探针Fluo-3/AM标记细胞,激光扫描共聚焦显微镜检测成骨细胞内游离钙浓度的变化情况.结果经10-4 mol/L P物质作用后,70%的成骨细胞发生了反应,细胞内游离钙浓度明显升高[荧光值增加(90±18)%].结论钙离子有可能作为细胞内第二信使,介导P物质对成骨细胞的调节作用.
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